Fig. 2
Extraembryonic MGA Controls BMP Signaling in the Embryo Embryos injected into the YSL with 4 ng of a 5 bp mismatch control MO, mgamisMO (A–D, I, J) or 4 ng mgaTL3MO (E–H, K, L). (A, B, E, and F) Live morphants at the prim-5 stage. (C and G) gata1 expression at the 8-somite stage. (D and H) egr2b expression at the 8-somite stage. (I and K) pSmad1/5 gradient at 75% epiboly. The inset (I) shows the YSL nuclei are labeled with nls-RFP. (J and L) bmp2b/swr expression at 75% epiboly. (M) Western blot of extracts of 75% epiboly embryos. In mgaMO>YSL morphants, pSmad1: 48% ± 2.8% SD of controls and pSmad5: 53.5% ± 16.2% SD of controls. In acvr1lMO>1-cell morphants, pSmad1: 25% ± 8.6% SD of controls and pSmad5: 21.25% ± 4.7% SD of controls. (N) Coimmunoprecipitation of HA-Smad4 with pSmad1/5. HA-smad1 mRNA was injected into the whole embryo at the 1-cell stage (lane 1) or into the YSL at 3 hpf (lane 2). Anterior is to the left in (A)–(H). Dorsal is to the right in (I)–(L). In all figures, fractions indicate the number of embryos with the depicted phenotype/total number injected. See also Figures S1 and S2. |
| Genes: | |
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| Antibody: | |
| Fish: | |
| Knockdown Reagent: | |
| Anatomical Terms: | |
| Stage Range: | 75%-epiboly to 5-9 somites |
| Fish: | |
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| Knockdown Reagent: | |
| Observed In: | |
| Stage Range: | 5-9 somites to Prim-5 |
Reprinted from Developmental Cell, 28(3), Sun, Y., Tseng, W.C., Fan, X., Ball, R., and Dougan, S.T., Extraembryonic signals under the control of MGA, Max, and Smad4 are required for dorsoventral patterning, 322-334, Copyright (2014) with permission from Elsevier. Full text @ Dev. Cell