FIGURE

Fig. 1

ID

ZDB-FIG-140211-10

Publication

Breau et al., 2013 - A Hox gene controls lateral line cell migration by regulating chemokine receptor expression downstream of Wnt signaling

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Fig. 1

hoxb8a is required for proper migration of the posterior lateral line primordium and expression of the chemokine receptor cxcr4b. Anterior to the left. Detection of hoxb8a mRNA (red) in cldnb:lyngfp embryos (green) at (A) 20, (B) 22, and (C) 35 hpf. Dorsal view in A, lateral views in B and C. White brackets indicate the width of the first forming proneuromast (L1). (D) Fuorescence images of cldnb:lyngfp embryos at 45 hpf injected with control and hoxb8a Mo. Red arrowheads indicate the position of the primordium. (E) Quantification of the migration of the primordium at 45 hpf (control Mo, n = 54; hoxb8a Mo, n = 91). ISH to detect expression of (F) cxcr4b, (G) cxcr7b, and (H) lef1 at 30 hpf. The primordium is outlined with dotted line. g, Posterior lateral line sensory ganglion; op, otic placode; nt, neural tube. (Scale bar: 25 µm.)

Expression Data

Genes:	ackr3b cxcr4b EGFP hoxb8a lef1
Fish:	WT WT + MO2-hoxb8a zf106Tg zf106Tg + MO2-hoxb8a
Knockdown Reagent:	MO2-hoxb8a
Anatomical Terms:	posterior lateral line ganglion posterior lateral line primordium posterior lateral line primordium posterior-most region protoneuromast
Stage Range:	20-25 somites to High-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	zf106Tg + MO2-hoxb8a
Knockdown Reagent:	MO2-hoxb8a
Observed In:	posterior lateral line neuromast primordium migration
Stage:	High-pec

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA