Fig. 6
- ID
- ZDB-FIG-131210-50
- Publication
- Zhen et al., 2013 - Hemogenic endothelium specification and hematopoietic stem cell maintenance employ distinct Scl isoforms
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scl-a7alpha; is required for the maintenance of HSCs in the AGM. (A-F) cmyb expression in control zebrafish embryos and scl-α morphants. Arrows indicate cmyb expression in AGM (A,B,D,E) or CHT (C,F) region. D, n=43/48; E, n=37/45; F, n=34/40. (G,H) CD41:eGFP+ HSCs in the AGM of 2 dpf control embryos and scl-7alpha; morphants (n=25/28). White arrows identify the CD41:eGFP+ HSCs and blue arrowheads indicate unaffected CD41:eGFP+ cells in the pronephric duct. (I-L2) Time-lapse confocal imaging of a live Tg(scl-α:DsRed; kdrl:eGFP) embryo injected with scl-α MO from <38 to 50 hpf. In J-L′, selected planes show three apoptotic HSCs in the scl-α morphants. Also see supplementary material Movie 4. Similar results were obtained in all four scl-α morphants observed (n=4/4). Scale bars: 50 μm in G,H; 20 μm in I-L2. |
| Genes: | |
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| Fish: | |
| Knockdown Reagent: | |
| Anatomical Terms: | |
| Stage Range: | Prim-5 to Protruding-mouth |
| Fish: | |
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| Knockdown Reagent: | |
| Observed In: | |
| Stage Range: | Prim-25 to Protruding-mouth |