ZFIN ID: ZDB-FIG-131210-39
Zhen et al., 2013 - Hemogenic endothelium specification and hematopoietic stem cell maintenance employ distinct Scl isoforms. Development (Cambridge, England)   140(19):3977-3985 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Prim-5 to Long-pec

Fig. 2 Expression of scl-β and of scl-α in the AGM represent two sequential cell populations. (A-C) Expression of fluorescent proteins in the AGM of a 26 hpf Tg(scl-β:d2eGFP; scl-α:DsRed) zebrafish embryo. The scl-β:d2eGFP signals were detected lining the ventral side of the dorsal aorta, whereas expression of scl-α:DsRed was absent. (D-F) Expression of fluorescent proteins in the AGM of a 25 hpf Tg(scl-β:d2eGFP; kdrl:Ras-mCherry) embryo. scl-β:d2eGFP was co-expressed with kdrl:Ras-mCherry in endothelial cells. (G-I) Expression of fluorescent proteins in the AGM of a 36 hpf Tg(scl-β:d2eGFP; scl-α:DsRed) embryo. The scl-β:d2eGFP and scl-α:DsRed signals overlapped in the forming HSCs. Arrows in A-I indicate the cells in the AGM that express the corresponding fluorescent proteins. (J-O) cmyb or CD41 (itga2b) is co-expressed with scl-α in AGM HSCs (arrowheads) of 48 hpf live Tg(cmyb:eGFP; scl-α:DsRed) embryos or 56 hpf live Tg(CD41:eGFP; scl-α:DsRed) embryos. Blue arrows indicate the direction of circulation. The red stripes in vessels are due to the rapid confocal scanning of circulating scl-α:DsRed+ red blood cells. DA, dorsal aorta; AV, axial vein. Scale bars: 20 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
d2EGFP hkz06tTg standard conditions Prim-5 ventral wall of dorsal aorta IFL
Prim-25 hematopoietic stem cell IFL
Prim-25 ventral wall of dorsal aorta hematopoietic stem cell IFL
hkz08tTg; s896Tg standard conditions Prim-5 endothelial cell IFL
DsRed hkz06tTg standard conditions Prim-5 Not Detected ventral wall of dorsal aorta IFL
Prim-25 hematopoietic stem cell IFL
Prim-25 ventral wall of dorsal aorta hematopoietic stem cell IFL
hkz07tTg; la2Tg standard conditions Long-pec nucleate erythrocyte IFL
Long-pec ventral wall of dorsal aorta hematopoietic stem cell IFL
hkz07tTg; zf169Tg standard conditions Long-pec nucleate erythrocyte IFL
Long-pec ventral wall of dorsal aorta hematopoietic stem cell IFL
EGFP hkz07tTg; la2Tg standard conditions Long-pec ventral wall of dorsal aorta hematopoietic stem cell IFL
hkz07tTg; zf169Tg standard conditions Long-pec ventral wall of dorsal aorta hematopoietic stem cell IFL
mCherry hkz08tTg; s896Tg standard conditions Prim-5 endothelial cell IFL
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development