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ZFIN ID: ZDB-FIG-131210-46
Zhen et al., 2013 - Hemogenic endothelium specification and hematopoietic stem cell maintenance employ distinct Scl isoforms. Development (Cambridge, England)   140(19):3977-3985 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Prim-5 to Long-pec
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Prim-5 to Long-pec

Fig. 4 scl-β is crucial for hemogenic endothelium specification. (A-D′) Time-lapse confocal imaging of a live Tg(scl-α:DsRed; kdrl:eGFP) scl-β zebrafish morphant from 25 to 36 hpf. White arrows and numbers indicate the endothelial cells that will undergo fragmentation during the following 6 hours. In B-D′, three kdrl:eGFP+ elongated endothelial cells burst into fragments without initiation of budding. Also see supplementary material Movie 3. Blue arrowheads in B indicate some cell debris that already existed before recording. Similar results were obtained in all three scl-β morphants observed (n=3/3). (E,F) CD41:eGFP+ HSCs in the AGM region of 2 dpf live control embryos and scl-β morphants. On average, fewer than one CD41:eGFP+ HSC could be detected in the AGM of scl-β morphants (n=17/19), compared with an average of 13 CD41:eGFP+ HSCs (white arrows) in the same region of control embryos. Blue arrowheads identify pronephric duct cells. Scale bars: 30 μm in A; 20 μm in B-F.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
DsRed hkz07tTg; s843Tg + MO8-tal1 standard conditions Prim-5 to Prim-15 endothelial cell IFL
Prim-5 to Prim-15 nucleate erythrocyte IFL
EGFP hkz07tTg; s843Tg + MO8-tal1 standard conditions Prim-5 to Prim-15 endothelial cell IFL
la2Tg control Long-pec pronephric duct cell IFL
Long-pec ventral wall of dorsal aorta hematopoietic stem cell IFL
la2Tg + MO8-tal1 standard conditions Long-pec pronephric duct cell IFL
Long-pec Not Detected ventral wall of dorsal aorta hematopoietic stem cell IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
hkz07tTg; s843Tg + MO8-tal1 standard conditions Prim-5 - Prim-15 ventral wall of dorsal aorta endothelial cell apoptotic, abnormal
la2Tg + MO8-tal1 standard conditions Long-pec ventral wall of dorsal aorta lacks all parts of type hematopoietic stem cell, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development