FIGURE

Fig. 7

ID

ZDB-FIG-131030-11

Publication

Lai et al., 2012 - Autotaxin/Lpar3 signaling regulates Kupffer's vesicle formation and left-right asymmetry in zebrafish

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Fig. 7

Targeted knockdown of lpar3 or atx in DFCs causes cardiac bifida in addition to laterality defects. (A) Zebrafish embryos at the 512- to 1000-cell stage were injected with 2.5 or 5 ng of the indicated MOs with or without 100 pg of lpar3 mRNA, as shown at the bottom of each column. Treated embryos were cultured until 26-28 hpf and then subjected to cardiac jogging analysis. The percentages of each phenotype in all treatments are shown in the lower graph. a-f, groups denoted with different lettering refer to statistical significance (P<0.05). (n=3). (B) Embryos with the indicated treatments were incubated to 48 hpf and then subjected to cardiac looping analysis.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO2-lpar3 AB + MO5-enpp2
Knockdown Reagents:	MO2-lpar3 MO5-enpp2
Observed In:	determination of heart left/right asymmetry heart heart jogging heart looping
Stage Range:	Prim-5 to Long-pec

Phenotype Detail

Acknowledgments

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