FIGURE

Fig. 3

ID

ZDB-FIG-130109-21

Publication

Lai et al., 2012 - Autotaxin/Lpar3 signaling regulates Kupffer's vesicle formation and left-right asymmetry in zebrafish

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Fig. 3

Kupffer′s vesicle formation is disturbed in both lpar3 and atx morphants. (A-J,N) Tg(sox17:dsRed) zebrafish embryos were injected with MO and the integrity of KV was examined under differential interference contrast (A-E) or epifluorescence (F-J) microscopy. In contrast to Std control MO-injected embryos (A,F), deformed (B,G,D,I) and/or reduced KVs (C,H,E,J) were observed in both lpar3 and atx morphants (open arrowheads). In addition, dispersed dsRed-positive cells (arrowheads) were also observed around KV in both lpar3 and atx morphants. To quantify KV defects, Tg(sox17:dsRed) embryos were used to repeat the above experiments and analyses are shown in N. (K-M,O) Embryos injected with the indicated MO were subjected to WISH against charon (cha, ventral view, bottom right). Arrowheads point to KVs. Magnified KV insets are shown at the lower right of each panel; embryo midlines are indicated by dashed lines. WISH against charon also revealed similar deformed/reduced pattern in lpar3 (L) and atx (M) morphants compared with Std control MO-injected embryos (K). Quantitative analyses are shown in O.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	s870Tg + MO2-lpar3 s870Tg + MO5-enpp2 s903Tg + MO2-lpar3 s903Tg + MO5-enpp2
Knockdown Reagents:	MO2-lpar3 MO5-enpp2
Observed In:	Kupffer's vesicle Kupffer's vesicle cell
Stage Range:	5-9 somites to 10-13 somites

Phenotype Detail

Acknowledgments

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