Sequence analysis and expression profile of zebrafish lpar3 gene. (A) Domain architecture of the G protein-coupled receptor Lpar3 in zebrafish. Lpar3 possesses evolutionarily conserved seven transmembrane domains (TMs) and translation-blocking MOs (tMO1 and tMO2) binding sites are indicated. Amino numbers flanking each transmembrane domain are shown. (B) Phylogenetic analysis of LPA receptors 1-3 from human (hLPAR1-3), mouse (mLpar1-3) and zebrafish (drLpar1-3). (C,D) WISH expression patterns (C) and RT-PCR analysis (D) of lpar3 in zebrafish embryos at the designated stages. ef1-α was used as an internal control.
|