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Fig. 7
Targeted knockdown of lpar3 or atx in DFCs causes cardiac bifida in addition to laterality defects. (A) Zebrafish embryos at the 512- to 1000-cell stage were injected with 2.5 or 5 ng of the indicated MOs with or without 100 pg of lpar3 mRNA, as shown at the bottom of each column. Treated embryos were cultured until 26-28 hpf and then subjected to cardiac jogging analysis. The percentages of each phenotype in all treatments are shown in the lower graph. a-f, groups denoted with different lettering refer to statistical significance (P<0.05). (n=3). (B) Embryos with the indicated treatments were incubated to 48 hpf and then subjected to cardiac looping analysis.