FIGURE

Fig. 5

ID

ZDB-FIG-130109-23

Publication

Lai et al., 2012 - Autotaxin/Lpar3 signaling regulates Kupffer's vesicle formation and left-right asymmetry in zebrafish

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Fig. 5

KV ciliogenesis is disturbed in lpar3 and atx morphants. (A-C) Cilia in KV for the indicated treatments were observed by immunofluorescence using anti-acetylated tubulin antibody (red) and counter-stained with aPKC antibody (green) to outline the KV. Representative photographs of Std MO-injected zebrafish embryos (A), lpar3 (B) and atx (C) morphants are presented (anterior side up). (D,E) Ten embryos were used for each treatment (three independent experiments) to measure cilia number (D) and volume (E). The number of embryos examined is presented in each column. Error bars represent s.e.m. a, b, groups denoted with different lettering refer to statistical significance (P<0.05).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO2-lpar3 AB + MO5-enpp2
Knockdown Reagents:	MO2-lpar3 MO5-enpp2
Observed In:	cilium assembly Kupffer's vesicle Kupffer's vesicle cilium
Stage:	5-9 somites

Phenotype Detail

Acknowledgments

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