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ZIRC
ZFIN ID: ZDB-FIG-130828-13
Zhao et al., 2013 - Reptin/Ruvbl2 is a Lrrc6/Seahorse interactor essential for cilia motility. Proceedings of the National Academy of Sciences of the United States of America   110(31):12697-702 Full text @ Proc. Natl. Acad. Sci. USA
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Term:
Stage: Long-pec
PHENOTYPE:
Fish:
Observed In:
Stage Range: Protruding-mouth to Day 5

Fig. 2

Reptin is essential for cilia motility. (A and B) Kymographs showing the rhythmic beating of a multicilia bundle in the posterior pronephric duct in a wild-type (WT) embryo and paralyzed cilia in a reptinhi2394 mutant (rep), or a slower beating cilium in a seahorsehi3308 mutant (sea) at 3 dpf. (C) Cilia beating frequency in wild-type (black bar) and seahorsehi3308 (white bar) pronephros at 3 dpf. Data are represented as mean + SD, from eight replicates. *P < 0.05. (D) Cilia beating frequency in the olfactory placode of wild-type (black bar, WT) and seahorsehi3308 mutant (white bar, sea) embryos at 3 dpf. Data are represented as mean + SD, from five replicates. *P < 0.05. (E) Electron micrographs of cross-sections of cilia in WT and reptinhi2394 mutants (rep) at 5 dpf. Outer and inner dynein arms are pointed out by white and black arrowheads, respectively. (F) Number of outer and inner dynein arms in WT and reptinhi2394 mutants (rep) per section at 5 dpf. Data are represented as mean + SD. **P < 0.01. (G) Expression levels of multiple factors involved in dynein arm formation as shown by quantitative PCR (qPCR). Y axis shows the fold changes in reptinhi2394 mutants. Data are represented as mean + SD, from three replicates. *P < 0.05, **P < 0.01. NS, nonsignificant.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
dnaaf1 ruvbl2hi2394Tg/hi2394Tg standard conditions Long-pec whole organism RTPCR
dnaaf2 ruvbl2hi2394Tg/hi2394Tg standard conditions Long-pec whole organism RTPCR
dnaaf3 ruvbl2hi2394Tg/hi2394Tg standard conditions Long-pec whole organism RTPCR
dnah9 ruvbl2hi2394Tg/hi2394Tg standard conditions Long-pec whole organism RTPCR
dnai1.1 ruvbl2hi2394Tg/hi2394Tg standard conditions Long-pec whole organism RTPCR
dnal1 ruvbl2hi2394Tg/hi2394Tg standard conditions Long-pec whole organism RTPCR
foxj1a ruvbl2hi2394Tg/hi2394Tg standard conditions Long-pec whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
lrrc6hi3308Tg/hi3308Tg standard conditions Protruding-mouth epithelial cilium movement involved in extracellular fluid movement decreased frequency, abnormal
Protruding-mouth olfactory epithelium motile cilium decreased speed, abnormal
Protruding-mouth posterior pronephric duct motile cilium decreased speed, abnormal
ruvbl2hi2394Tg/hi2394Tg standard conditions Protruding-mouth epithelial cilium movement involved in extracellular fluid movement arrested, abnormal
Protruding-mouth posterior pronephric duct motile cilium immobile, abnormal
Day 5 inner dynein arm assembly decreased occurrence, abnormal
Day 5 outer dynein arm assembly decreased occurrence, abnormal
Day 5 pronephric duct motile cilium has fewer parts of type pronephric duct inner dynein arm, abnormal
Day 5 pronephric duct motile cilium has fewer parts of type pronephric duct outer dynein arm, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Proceedings of the National Academy of Sciences of the United States of America for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Proc. Natl. Acad. Sci. USA