ZFIN ID: ZDB-FIG-130815-2
Choudhuri et al., 2013 - Translation initiation factor eIF3h targets specific transcripts to polysomes during embryogenesis. Proc. Natl. Acad. Sci. USA   110(24):9818-9823 Full text @ Proc. Natl. Acad. Sci. USA
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage: Prim-5

Fig. 2

Validation of the change in translation state for genes identified through RNA-seq. Shown are qPCR results from three independent biological isolates of both WT and eif3ha morphants for 25 genes that had been found to be decreased in translational state (TS) and five randomly chosen genes that had shown no change in TS. The bars represent the mean and the error bars the SEM. The change in TS has been calculated using the formula [(WT/MO)poly/[(WT/MO)total], where (WT/MO)poly and (WT/MO)total represent the changes of individual mRNAs in polysomal and total RNA, respectively. A negative sign is added arbitrarily to the calculated values to indicate that the translation efficiency is reduced in the morphants relative to WT. Actual data for each independent set, compared with the RNA-seq data, are presented in Table S2.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
and2 AB/TU + MO3-eif3ha standard conditions Prim-5 whole organism polysome RTPCR
and3 AB/TU + MO3-eif3ha standard conditions Prim-5 whole organism polysome RTPCR
atp2a1l AB/TU + MO3-eif3ha standard conditions Prim-5 whole organism polysome RTPCR
bscl2l AB/TU + MO3-eif3ha standard conditions Prim-5 whole organism polysome RTPCR
cox4i1l AB/TU + MO3-eif3ha standard conditions Prim-5 whole organism polysome RTPCR
cryba1l2 AB/TU + MO3-eif3ha standard conditions Prim-5 whole organism polysome RTPCR
crygm2d2 AB/TU + MO3-eif3ha standard conditions Prim-5 whole organism polysome RTPCR
crygm2d5 AB/TU + MO3-eif3ha standard conditions Prim-5 whole organism polysome RTPCR
crygm2d8 AB/TU + MO3-eif3ha standard conditions Prim-5 whole organism polysome RTPCR
crygm2d9 AB/TU + MO3-eif3ha standard conditions Prim-5 whole organism polysome RTPCR
crygm2d10 AB/TU + MO3-eif3ha standard conditions Prim-5 whole organism polysome RTPCR
crygm2d11 AB/TU + MO3-eif3ha standard conditions Prim-5 whole organism polysome RTPCR
crygm2d13 AB/TU + MO3-eif3ha standard conditions Prim-5 whole organism polysome RTPCR
crygm2d17 AB/TU + MO3-eif3ha standard conditions Prim-5 whole organism polysome RTPCR
crygm2d18 AB/TU + MO3-eif3ha standard conditions Prim-5 whole organism polysome RTPCR
crygm2d19 AB/TU + MO3-eif3ha standard conditions Prim-5 whole organism polysome RTPCR
crygm2d21 AB/TU + MO3-eif3ha standard conditions Prim-5 whole organism polysome RTPCR
crygmxl2 AB/TU + MO3-eif3ha standard conditions Prim-5 whole organism polysome RTPCR
fgl1 AB/TU + MO3-eif3ha standard conditions Prim-5 whole organism polysome RTPCR
hspb2 AB/TU + MO3-eif3ha standard conditions Prim-5 whole organism polysome RTPCR
mipb AB/TU + MO3-eif3ha standard conditions Prim-5 whole organism polysome RTPCR
pvalb1 AB/TU + MO3-eif3ha standard conditions Prim-5 whole organism polysome RTPCR
si:dkey-17e16.15 AB/TU + MO3-eif3ha standard conditions Prim-5 whole organism polysome RTPCR
slc18a3b AB/TU + MO3-eif3ha standard conditions Prim-5 whole organism polysome RTPCR
slc25a4 AB/TU + MO3-eif3ha standard conditions Prim-5 whole organism polysome RTPCR
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Proc. Natl. Acad. Sci. USA for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Proc. Natl. Acad. Sci. USA