FIGURE

Fig. 2

ID

ZDB-FIG-130815-2

Publication

Choudhuri et al., 2013 - Translation initiation factor eIF3h targets specific transcripts to polysomes during embryogenesis

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Fig. 2

Validation of the change in translation state for genes identified through RNA-seq. Shown are qPCR results from three independent biological isolates of both WT and eif3ha morphants for 25 genes that had been found to be decreased in translational state (TS) and five randomly chosen genes that had shown no change in TS. The bars represent the mean and the error bars the SEM. The change in TS has been calculated using the formula [(WT/MO)_poly/[(WT/MO)_total], where (WT/MO)_poly and (WT/MO)_total represent the changes of individual mRNAs in polysomal and total RNA, respectively. A negative sign is added arbitrarily to the calculated values to indicate that the translation efficiency is reduced in the morphants relative to WT. Actual data for each independent set, compared with the RNA-seq data, are presented in Table S2.

Expression Data

Genes:	and2 and3 atp2a1l bscl2l cox4i1l cryba1l2 crygm2d2 crygm2d5 crygm2d8 crygm2d9 crygm2d10 crygm2d11 crygm2d13 crygm2d17 crygm2d18 crygm2d19 crygm2d21 crygmxl2 fgl1 hspb2 mipb pvalb1 si:dkey-17e16.15 slc18a3b slc25a4
Fish:	AB/TU + MO3-eif3ha
Knockdown Reagent:	MO3-eif3ha
Anatomical Term:	whole organism ribosome
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB/TU + MO3-eif3ha
Knockdown Reagent:	MO3-eif3ha
Observed In:	whole organism ribosome
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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