FIGURE

Fig. 4

ID

ZDB-FIG-130802-39

Publication

Choudhuri et al., 2013 - Translation initiation factor eIF3h targets specific transcripts to polysomes during embryogenesis

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Fig. 4

Crystallins of the crygm2d family are a cohort of genes that are translationally regulated by eif3ha. (A) Transcripts encoding crygm2d isoforms are shown according to their respective changes in the translation state relative to total RNA. (B) The change in translational state was validated in independent experiments by qPCR for crygm2d3, crygm2d4, and crygm2d12. (C) Representative in situ hybridization experiment showing that crygm2d1, crygm2d12, and eif3ha transcripts are colocalized in the developing lens at 1–2 dpf. (D) Representative in situ hybridization experiment showing that transcript patterns for crygm2d3 and crygm2d12 are unaltered in eif3ha morphants at 24 hpf compared with stage-matched WT controls.

Expression Data

Genes:	crygm2d1 crygm2d3 crygm2d4 crygm2d12
Fish:	AB/TU AB/TU + MO3-eif3ha WT + MO3-eif3ha
Knockdown Reagent:	MO3-eif3ha
Anatomical Terms:	lens solid lens vesicle whole organism ribosome
Stage Range:	Prim-5 to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB/TU + MO3-eif3ha
Knockdown Reagent:	MO3-eif3ha
Observed In:	whole organism ribosome
Stage:	Prim-5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA