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ZFIN ID: ZDB-FIG-130801-34
Wang et al., 2013 - miR-34b regulates multiciliogenesis during organ formation in zebrafish. Development (Cambridge, England)   140(13):2755-2764 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Term:
Stage Range: Prim-5 to Prim-25
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Prim-25

Fig. 7 cmyb regulates the expression of key regulatory genes involved in centriole duplication and ciliogenesis. (A-D) Morphological and statistical analysis of the centriole (anti-γ-tubulin staining, green in A) and cilia bundle (anti-acetylated tubulin staining, green in B) in the middle kidney duct of wild-type and cmyb mutant embryos. Membrane RFP was injected and stained to help identify the cell boundaries (data not shown). Arrows indicate normal centrioles and ciliar bundles; arrowheads indicate defective centrioles and cilia bundles. (E) The percentage of cmyb:EGFP+ cells with cilia bundles was calculated for each Tg(hsp70:cmyb) embryo and analyzed statistically at 36 hpf. (C-E) Each circle/triangle represents an embryo. Mean ± s.d. **P<0.01, ***P<0.0001 (unpaired t-test). (F,G) WISH analysis of plk4, stil, rfx2 and foxj1b expression in normal embryos, cmyb mutants and embryos overexpressing cmyb. Arrows indicate the regions where MCCs are located. The number of embryos with normal (top pictures in F,G), low (bottom pictures in F) or high expression levels (bottom pictures in G) of a tested gene among all analyzed embryos is indicated (bottom right). WT, wild type. Scale bars: 10 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
foxj1b WT standard conditions Prim-5 pronephros multi-ciliated epithelial cell ISH
hkz03tTg standard conditions Prim-25 pronephros multi-ciliated epithelial cell ISH
hkz03tTg heat shock Prim-25 pronephros multi-ciliated epithelial cell ISH
mybhkz3/hkz3 standard conditions Prim-5 pronephros multi-ciliated epithelial cell ISH
plk4 WT standard conditions Prim-5 pronephros multi-ciliated epithelial cell ISH
hkz03tTg standard conditions Prim-25 pronephros multi-ciliated epithelial cell ISH
hkz03tTg heat shock Prim-25 pronephros multi-ciliated epithelial cell ISH
mybhkz3/hkz3 standard conditions Prim-5 pronephros multi-ciliated epithelial cell ISH
rfx2 WT standard conditions Prim-5 pronephros multi-ciliated epithelial cell ISH
hkz03tTg standard conditions Prim-25 pronephros multi-ciliated epithelial cell ISH
hkz03tTg heat shock Prim-25 pronephros multi-ciliated epithelial cell ISH
mybhkz3/hkz3 standard conditions Prim-5 pronephros multi-ciliated epithelial cell ISH
stil WT standard conditions Prim-5 pronephros multi-ciliated epithelial cell ISH
hkz03tTg standard conditions Prim-25 pronephros multi-ciliated epithelial cell ISH
hkz03tTg heat shock Prim-25 pronephros multi-ciliated epithelial cell ISH
mybhkz3/hkz3 standard conditions Prim-5 pronephros multi-ciliated epithelial cell ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
hkz03tTg heat shock Prim-25 multi-ciliated epithelial cell centriole displaced to multi-ciliated epithelial cell cytoplasm, abnormal
mybhkz3/hkz3 standard conditions Prim-25 multi-ciliated epithelial cell cilium physical object quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development