ZFIN ID: ZDB-FIG-130801-29
Wang et al., 2013 - miR-34b regulates multiciliogenesis during organ formation in zebrafish. Development (Cambridge, England)   140(13):2755-2764 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Protruding-mouth

Fig. 2 Loss of miR-34b disrupts kidney morphogenesis. (A-F) In situ hybridization with the rfx2 probe and images of the Tg(cmyb:EGFP) embryo show that MCCs accumulate in the middle part of the pronephric duct (arrows) in miR-34b morphants at 3 dpf. C and F show the percentage of embryos with abnormal cmyb+ (shown in A,B) or rfx2+ (shown in D,E) MCCs. Embryos with accumulated MCCs (Accumulated) or higher expression levels of cmyb or rfx2 (Higher expression) were counted. (G-I) A magnified lateral view (with a small angle to dorsal) of a 3-dpf embryo after in situ hybridization with the pax2 probe shows that proximal tubule convolution is blocked at 3 dpf in miR-34b morphants. Examples of normal proximal tubule convolution (G) and of mild and severe defects (H) are illustrated in the insets. The angle between the kidney duct and tubule (DT angle) was measured. Each circle/triangle in I represents an embryo. Mean ± s.d. ***P<0.0001 (unpaired t-test). (J-L) Lateral view of a 3-dpf embryo after in situ hybridization with the trpm7 probe shows that transporting epithelial cells accumulate in the middle region (bracket) of the pronephric duct 3 dpf after miR-34b knockdown. L shows the percentage of embryos with abnormal distribution of trpm7+ transporting epithelial cells (Defective). (M,N) Hematoxylin and Eosin (H&E) staining on a transverse section (plane indicated by the white line in A) shows the enlarged tubule diameter (outlined) after miR-34b knockdown.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP zf169Tg standard conditions Protruding-mouth pronephros multi-ciliated epithelial cell IHC
zf169Tg + MO1-mir34b standard conditions Protruding-mouth pronephros multi-ciliated epithelial cell IHC
pax2a WT standard conditions Protruding-mouth pronephros epithelium ISH
WT + MO1-mir34b standard conditions Protruding-mouth pronephros epithelium ISH
rfx2 WT standard conditions Protruding-mouth pronephros multi-ciliated epithelial cell ISH
WT + MO1-mir34b standard conditions Protruding-mouth pronephros multi-ciliated epithelial cell ISH
trpm7 WT standard conditions Protruding-mouth pronephros epithelial cell ISH
WT + MO1-mir34b standard conditions Protruding-mouth pronephros epithelial cell ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-mir34b standard conditions Protruding-mouth multi-ciliated epithelial cell accumulation pronephric duct, abnormal
Protruding-mouth pronephric proximal convoluted tubule closed, abnormal
Protruding-mouth pronephric tubule increased size, abnormal
Protruding-mouth pronephros epithelial cell physical object quality, abnormal
Protruding-mouth pronephros epithelial cell shortened, abnormal
zf169Tg + MO1-mir34b standard conditions Protruding-mouth multi-ciliated epithelial cell accumulation pronephric duct, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development