ZFIN ID: ZDB-FIG-130801-30
Wang et al., 2013 - miR-34b regulates multiciliogenesis during organ formation in zebrafish. Development (Cambridge, England)   140(13):2755-2764 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Prim-25 to Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Long-pec to Protruding-mouth

Fig. 3 MCC accumulation is mainly caused by attenuated cell migration. (A) A magnified lateral view of the kidney region after double staining of cmyb:EGFP and PH3 in 3-dpf embryos. (B) A magnified lateral view of the kidney region after TUNEL staining in 3-dpf embryos. (C,D) Distribution of transporting epithelial cells (bracket) (C) and MCCs (D) labeled by trpm7 (WISH) and Tg(cmyb:EGFP) at 36 hpf. (B-D) The number of embryos with normal TUNEL staining, trpm7 expression pattern or cmyb expression pattern among all analyzed embryos is indicated (bottom right). (E-G) The migration of MCCs labeled by Tg(cmyb:EGFP) from 51 to 72 hpf. Three representative MCCs were labeled (arrowheads and brackets) in embryos injected with either control (Ctrl) MO (E) or miR-34b MO (F). The migration distance of each MCC is shown in G (each circle/triangle represents an MCC, n=10). Mean ± s.d. ***P<0.0001 (unpaired t-test); ns, not significant. Scale bar: 100 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP zf169Tg standard conditions Prim-25 pronephros multi-ciliated epithelial cell IHC
Long-pec to Protruding-mouth pronephros multi-ciliated epithelial cell IFL
Protruding-mouth pronephros multi-ciliated epithelial cell IHC
zf169Tg + MO1-mir34b standard conditions Prim-25 pronephros multi-ciliated epithelial cell IHC
Long-pec to Protruding-mouth pronephros multi-ciliated epithelial cell IFL
Protruding-mouth pronephros multi-ciliated epithelial cell IHC
trpm7 WT standard conditions Prim-25 pronephros epithelial cell ISH
WT + MO1-mir34b standard conditions Prim-25 pronephros epithelial cell ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
zf169Tg + MO1-mir34b standard conditions Long-pec - Protruding-mouth multi-ciliated epithelial cell cell migration involved in kidney development decreased process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development