ZFIN ID: ZDB-FIG-130801-31
Wang et al., 2013 - miR-34b regulates multiciliogenesis during organ formation in zebrafish. Development (Cambridge, England)   140(13):2755-2764 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Prim-25 to Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Prim-25 to Protruding-mouth

Fig. 4 miR-34b regulates kidney morphogenesis by controlling multiciliogenesis and fluid flow. (A) Heat map showing the expression of genes in the GO term Cilium Assembly. The color scale represents the expression level of a gene above (red) or below (green) the mean expression level across all samples. (B) QRT-PCR analysis of the expression of cilia genes in MCCs that were isolated from 3-dpf Tg(cmyb:EGFP) embryos by flow cytometry. The expression levels were normalized to that of β-actin 1. (C) The ciliary (anti-acetylated tubulin staining, red, arrows) morphology of the MCCs (anti-GFP staining, green) at 36 hpf in normal embryos and miR-34b morphants. Arrows indicate normal cilia bundles. (D) The percentage of cmyb:EGFP+ cells with cilia bundles was calculated for each embryo and analyzed statistically at 36 hpf. (E) The ciliary morphology of the olfactory placode (outlined) at 3 dpf in normal embryos and miR-34b morphants. The number of embryos with normal cilia (top picture) or shorter cilia (bottom picture) among all analyzed embryos is indicated (bottom left). (F) Representative example of the dye excretion assay (3.5-dpf normal embryo), in which the time from injection until the first visible urine excretion at the cloaca (arrow) is measured. (G) Statistical analysis of the time from injection to dye excretion. Each circle/triangle represents an embryo. Mean ± s.d. ***P<0.0001 (unpaired t-test). Scale bar: 10 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP zf169Tg standard conditions Prim-25 pronephros multi-ciliated epithelial cell IHC
zf169Tg + MO1-mir34b standard conditions Prim-25 pronephros multi-ciliated epithelial cell IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-tuba IHC WT standard conditions Protruding-mouth peripheral olfactory organ cilium
IHC WT + MO1-mir34b standard conditions Protruding-mouth peripheral olfactory organ cilium
IHC zf169Tg standard conditions Prim-25 pronephros multi-ciliated epithelial cell
IHC zf169Tg + MO1-mir34b standard conditions Prim-25 pronephros multi-ciliated epithelial cell
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-mir34b standard conditions Protruding-mouth olfactory epithelium cilium shortened, abnormal
zf169Tg + MO1-mir34b standard conditions Prim-25 multi-ciliated epithelial cell cilium disorganized, abnormal
Prim-25 multi-ciliated epithelial cell cilium disoriented, abnormal
Prim-25 multi-ciliated epithelial cell cilium physical object quality, abnormal
Prim-25 pronephros multi-ciliated epithelial cell lacks parts or has fewer parts of type multi-ciliated epithelial cell cilium, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development