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ZIRC
ZFIN ID: ZDB-FIG-130725-20
Helker et al., 2013 - The zebrafish common cardinal veins develop by a novel mechanism: lumen ensheathment. Development (Cambridge, England)   140(13):2776-2786 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: Prim-5 to Long-pec
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Prim-15 to Long-pec

Fig. 8 Vegfc expressed from erythrocytes regulates EC numbers in the CCV. (A-C) Expression of vegfc in the region of the heart (arrows) in embryos injected with control MO (A), pu.1 MO (B) or gata1 MO (C) detected by in situ hybridization at 32 hpf; lateral views. (D,E) Quantification of EC numbers in the CCVs at 32 hpf (D) and 48 hpf (E). The number of ECs increased in embryos lacking macrophages (pu.1 MO-injected, D) and decreased in embryos lacking erythrocytes (gata1 MO-injected, D) or blood flow (tnnt2a MO-injected, E). (F-H) Confocal projections of Tg(etsrp:EGFP)ci1 CCV (F) and intersegmental vessel (Se) (G) at 44 hpf (lateral views) and comparison of the lumen diameter (H). The lumen diameter in the dorsal CCV is seven times larger and in the ventral CCV 20 times larger than that of the intersegmental vessel. Scale bars: 30 μm. (I) ECs start to proliferate with the onset of circulation. Until 24 hpf, ECs in the CCV still express the angioblast marker etsrp and only 5% proliferate. With the onset of circulation, proliferation of CCV ECs increases to 20%. Embryos were pulsed with BrdU at the indicated time points. (J) RT-PCR analysis of erythrocytes isolated from Tg(gata1:DsRed)sd2 embryos at 24 hpf and 48 hpf. gata1:DsRed positive (+) and negative (-) cells were isolated by FACS and analyzed for β-actin, gata1 and vegfc expression. **P<0.01, ***P<0.001; error bars indicate s.e.m.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
EGFP ci1Tg standard conditions High-pec common cardinal vein blood vessel endothelial cell IFL
High-pec intersegmental vessel blood vessel endothelial cell IFL
gata1a WT standard conditions Prim-5 Not Detected nucleate erythrocyte RTPCR
Long-pec Not Detected nucleate erythrocyte RTPCR
sd2Tg standard conditions Prim-5 nucleate erythrocyte RTPCR
Long-pec nucleate erythrocyte RTPCR
vegfc WT control Prim-15 nucleate erythrocyte ISH
WT standard conditions Prim-5 nucleate erythrocyte RTPCR
Long-pec nucleate erythrocyte RTPCR
WT + MO1-gata1a standard conditions Prim-15 Not Detected nucleate erythrocyte ISH
WT + MO1-spi1b standard conditions Prim-15 nucleate erythrocyte ISH
sd2Tg standard conditions Prim-5 Not Detected nucleate erythrocyte RTPCR
Long-pec nucleate erythrocyte RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-gata1a standard conditions Prim-15 common cardinal vein blood vessel endothelial cell decreased amount, abnormal
Prim-15 nucleate erythrocyte absent, abnormal
Prim-15 nucleate erythrocyte development disrupted, abnormal
WT + MO1-spi1b standard conditions Prim-15 common cardinal vein blood vessel endothelial cell increased amount, abnormal
Prim-15 macrophage decreased amount, abnormal
WT + MO1-tnnt2a standard conditions Long-pec common cardinal vein blood vessel endothelial cell decreased amount, abnormal
Long-pec nucleate erythrocyte development disrupted, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development