Fig. S3
ECs of the CCV form tight and adherens junctions. (A-C) Fluorescent confocal images of immunostainings of the junctional proteins ZO-1 and Cdh5 at the indicated time points; lateral views. The developing vasculature is visualized by transgenic EGFP expression of Tg(fli1a:EGFP)y1. (A-A′′) ECs of the CCV express the tight junction protein ZO-1 and the adherens junction protein Cdh5 at 26 hpf. (B-B′′) ECs of the CCV express the tight junction protein ZO-1 and the adherens junction protein Cdh5 at 32 hpf. (C-C′′) ECs of the CCV express the tight junction protein ZO-1 and the adherens junction protein Cdh5 at 48 hpf. Note that the epidermis shows nonspecific Cdh5 staining at 48 hpf. |