FIGURE

Fig. 4

ID

ZDB-FIG-130516-31

Publication

Schuler et al., 2013 - Ciliated sensory hair cell formation and function require the F-BAR protein syndapin I and the WH2 domain-based actin nucleator Cobl

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Fig. 4

Knock-down of either cobl or syndapin I diminishes neuromasts of the posterior lateral line organ. (A,B) Wild-type zebrafish (72hpf) analyzed for cobl and syndapin I expression patterns by in situ hybridization. Arrowheads mark regular arrays of cobl- and syndapin I-positive spots in the tails. (C?G) In situ hybridization of eya1 (neuromast marker) at 48hpf. Neuromasts at the facing tail side are marked by arrowheads. (D?G) Knock-down of cobl (D,E) and syndapin I (F,G) leads to reduced neuromast numbers. (H) Quantitative analysis. Wild-type, n = 117; MO p53, n = 55; MO sdpI_e3i3, n = 27; MO sdpI_ATG, n = 58; MOs sdpI_ATG+p53, n = 17; MO cobl_e4i4, n = 16; MO cobl_i3e4, n = 48; MOs cobl_i3e4+p53, n = 26. ANOVA with Tukey?s test versus MO p53; **P<0.01 for MO sdpI_e3i3 and ***P<0.001 for all other data sets. PLL, posterior lateral line.

Expression Data

Genes:	cobl eya1 pacsin1b
Fish:	AB AB + MO2-pacsin1b AB + MO3-cobl AB + MO3-pacsin1b AB + MO4-cobl
Knockdown Reagents:	MO2-pacsin1b MO3-cobl MO3-pacsin1b MO4-cobl
Anatomical Terms:	neuromast post-vent region trunk
Stage Range:	Long-pec to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO2-pacsin1b AB + MO3-cobl AB + MO3-pacsin1b AB + MO4-cobl
Knockdown Reagents:	MO2-pacsin1b MO3-cobl MO3-pacsin1b MO4-cobl
Observed In:	posterior lateral line neuromast
Stage:	Long-pec

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Cell Sci.