FIGURE

Fig. 3

ID

ZDB-FIG-130516-30

Publication

Schuler et al., 2013 - Ciliated sensory hair cell formation and function require the F-BAR protein syndapin I and the WH2 domain-based actin nucleator Cobl

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Fig. 3

Cobl and syndapin I morphants are paralyzed or display uncoordinated movements and fail to keep their balance. (A?C) Dechorionated control-injected embryos (48hpf) show typical directed escape responses after tactile stimulation (A), whereas syndapin I (B) and cobl (C) morphants did not respond or moved in an uncoordinated spinning manner. The swim paths of moving fish (numbered) are depicted. Note that both cobl- and syndapin I-morphant fish also often lie on their side. Scale bars: 5mm. (D) Quantification of the percentage of fish showing uncoordinated spinning behavior [control-injected (MO p53), n = 28; MO sdpI_ATG, n = 18; MO cobl_i3e4, n = 77 animals]. (E) Quantitative analysis of balance keeping after tactile stimulation. SdpI- and cobl-morphant embryos lie on their sides and fail to orientate upright (MO p53, n = 10; MO sdpI_ATG, n = 40; MO cobl_i3e4, n = 24; two independent experiments at 48 hpf).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO2-pacsin1b AB + MO3-cobl
Knockdown Reagents:	MO2-pacsin1b MO3-cobl
Observed In:	neuromuscular process controlling balance swimming behavior
Stage:	Long-pec

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Cell Sci.