FIGURE

Fig. 6

ID

ZDB-FIG-130516-33

Publication

Schuler et al., 2013 - Ciliated sensory hair cell formation and function require the F-BAR protein syndapin I and the WH2 domain-based actin nucleator Cobl

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Fig. 6

Both F-actin-rich stereocilia and microtubule-based kinocilia are defective in cobl and sdpI morphants. (A–D) SEM images of hair cells of neuromasts of zebrafish embryos injected as indicated at 72hpf. In both cobl- (B) and sdpI-morphant fish (C,D), kinocilia are reduced in number and significantly shortened. Tips of kinocilia are marked by asterisks. F-actin-rich stereocilia (arrows in A–D mark examples) also are severely affected. Scale bar: 1μm. (E,F) Quantitative analyses of observable stereocilia (E) and kinocilia (F). Wild-type, n = 99 kinocilia/82 stereocilia; control MO p53, n = 80 kinocilia/17 stereocilia; MO sdpI_e3i3, n = 37 kinocilia/38 stereocilia; MO sdpI_ATG, n = 10 kinocilia/5 stereocilia; MO cobl_i3e4, n = 58 kinocilia/19 stereocilia. Statistical analyses were performed versus wild-type. *P<0.05, **P<0.01, ***P<0.001. Kinocilia defects observed in cobl and syndapin I morphants (F) show even higher statistical significances versus MO p53-control (not depicted), MO sdpI_e3i3 (**), MO sdpI_ATG (***) and MO cobl_i3e4 (***).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO2-pacsin1b AB + MO3-cobl AB + MO3-pacsin1b
Knockdown Reagents:	MO2-pacsin1b MO3-cobl MO3-pacsin1b
Observed In:	neuromast kinocilium posterior lateral line neuromast posterior lateral line neuromast kinocilium posterior lateral line neuromast stereocilium
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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