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ZFIN ID: ZDB-FIG-130228-29
Cooney et al., 2013 - Teleost growth factor independence (gfi) genes differentially regulate successive waves of hematopoiesis. Developmental Biology   373(2):431-441 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 20-25 somites to Prim-25

Fig. 6 gfi1aa and gfi1b function at different hierarchical levels in hematopoietic epistasis. (A) The ICM expression of gfi1aa and gfi1b was evaluated in mutants with genetic blocks at sequential stages in hematopoiesis at the 20 ss. (a–b) Both gfi1aa and gfi1b are expressed in the ICM (arrowheads) in wild type embryos. (c–d) Mutants with genetic defects at the hemangioblast level (clo) and hematopoietic progenitors (scl) specifically lack ICM expression of either gfi1aa or gfi1b. Note that the neural expression of the gfi genes is preserved in these genetic mutants, confirming the specificity of the assay. (g–h) ICM expression of gfi1aa is preserved in the gata1 mutant, vlt, whereas gfi1b expression is deficient. (i–j) In contrast, both gfi1aa and gfi1b are expressed in the ICM of the mfrn1 mutant, frs. (B) To confirm the presence of hematopoietic progenitors in various genetic mutants, the expression of lmo2 and gata2 was evaluated in mutant embryos at the 20 ss. (a–b) Both lmo2 and gata2 are expressed in the ICM of wild type control embryos. (c–d) Neither lmo2 nor gata2 is expressed in the ICM of clo mutants, which fail to specify hematopoietic and vascular progenitors. (e–h) Expression of lmo2 and gata2 is preserved in scl and gata1 (vlt) mutants, confirming that hematopoietic progenitors are present. (C) The AGM expression of ikaros, gfi1aa and gfi1b was evaluated in runx-1 mutants at 36 hpf and embryos were genotyped for runx-1 wild type and mutant alleles. ikaros was included as a reference control. (a–b) AGM expression (brackets) of ikaros is silenced in runx-1 mutants relative to wild type embryos (wt), confirming the defect in definitive hematopoiesis. (c–d) AGM expression of gfi1aa is preserved in runx-1 mutants. (e–f) Loss of runx-1 silences expression of gfi1b in the AGM.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
gata2a WT standard conditions 20-25 somites intermediate cell mass of mesoderm hematopoietic stem cell ISH
gata1am651/m651 standard conditions 20-25 somites intermediate cell mass of mesoderm hematopoietic stem cell ISH
npas4lDf(Chr13:lycat)m39 standard conditions 20-25 somites Not Detected intermediate cell mass of mesoderm hematopoietic stem cell ISH
tal1t21384/t21384 standard conditions 20-25 somites intermediate cell mass of mesoderm hematopoietic stem cell ISH
gfi1aa WT standard conditions 20-25 somites intermediate cell mass of mesoderm ISH
Prim-25 ventral wall of dorsal aorta ISH
gata1am651/m651 standard conditions 20-25 somites central nervous system ISH
20-25 somites intermediate cell mass of mesoderm ISH
npas4lDf(Chr13:lycat)m39 standard conditions 20-25 somites central nervous system ISH
20-25 somites Not Detected intermediate cell mass of mesoderm ISH
runx1hg1/hg1 standard conditions Prim-25 ventral wall of dorsal aorta ISH
slc25a37tq223/tq223 standard conditions 20-25 somites central nervous system ISH
20-25 somites intermediate cell mass of mesoderm ISH
tal1t21384/t21384 standard conditions 20-25 somites central nervous system ISH
20-25 somites Not Detected intermediate cell mass of mesoderm ISH
gfi1b WT standard conditions 20-25 somites intermediate cell mass of mesoderm ISH
Prim-25 ventral wall of dorsal aorta ISH
gata1am651/m651 standard conditions 20-25 somites central nervous system ISH
20-25 somites Not Detected intermediate cell mass of mesoderm ISH
npas4lDf(Chr13:lycat)m39 standard conditions 20-25 somites central nervous system ISH
20-25 somites Not Detected intermediate cell mass of mesoderm ISH
runx1hg1/hg1 standard conditions Prim-25 Not Detected ventral wall of dorsal aorta ISH
slc25a37tq223/tq223 standard conditions 20-25 somites central nervous system ISH
20-25 somites intermediate cell mass of mesoderm ISH
tal1t21384/t21384 standard conditions 20-25 somites central nervous system ISH
20-25 somites Not Detected intermediate cell mass of mesoderm ISH
ikzf1 WT control Prim-25 ventral wall of dorsal aorta ISH
runx1hg1/hg1 standard conditions Prim-25 Not Detected ventral wall of dorsal aorta ISH
lmo2 WT standard conditions 20-25 somites intermediate cell mass of mesoderm hematopoietic stem cell ISH
gata1am651/m651 standard conditions 20-25 somites intermediate cell mass of mesoderm hematopoietic stem cell ISH
npas4lDf(Chr13:lycat)m39 standard conditions 20-25 somites Not Detected intermediate cell mass of mesoderm hematopoietic stem cell ISH
tal1t21384/t21384 standard conditions 20-25 somites intermediate cell mass of mesoderm hematopoietic stem cell ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 373(2), Cooney, J.D., Hildick-Smith, G.J., Shafizadeh, E., McBride, P.F., Carroll, K.J., Anderson, H., Shaw, G.C., Tamplin, O.J., Branco, D.S., Dalton, A.J., Shah, D.I., Wong, C., Gallagher, P.G., Zon, L.I., North, T.E., and Paw, B.H., Teleost growth factor independence (gfi) genes differentially regulate successive waves of hematopoiesis, 431-441, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.