ZFIN ID: ZDB-FIG-130222-17
Cooney et al., 2013 - Teleost growth factor independence (gfi) genes differentially regulate successive waves of hematopoiesis. Developmental Biology   373(2):431-441 Full text @ Dev. Biol.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: 20-25 somites

Fig. 3 gfi1aa regulates the primitive wave of zebrafish hematopoiesis. (A) gfi morphants and matching controls were subjected to WISH and analyzed for ICM expression (arrowhead) of primitive hematopoietic markers at the 20 ss. (a–f) ICM expression of the early hematopoietic markers, lmo2 and gata2, is preserved in gfi morphants and controls. (g–l) gfi1aa morphants show a reduction in ICM expression of scl and gata1, markers of primitive hematopoietic progenitors as compared to controls. Loss of gfi1b does not affect scl or gata1 expression in primitive hematopoiesis (c, f, i, l). (m–o) Loss of gfi1aa shows an expansion in the population of pu.1 myeloid progenitors, while no change is observed in gfi1b morphants. (p–r) The mpo myeloid population is expanded in gfi1aa morphants. (B) Transgenic reporter fish for primitive wave hematopoietic progenitors were subjected to FACS analysis after morpholino-mediated knockdown. (a) FACS of Tg(gata1:eGFP) embryos at the 20 ss reveals a significant decrease in the population of erythroid progenitors in gfi1aa morphants relative to controls. Erythroid progenitors are unaffected in gfi1b morphants (mean±SE, t test, p<0.05, n=3). (b) FACS of Tg (pu.1:eGFP) embryos at the 20 ss reveals a significant increase in the population of myeloid progenitors in gfi1aa morphants relative to controls. Myeloid progenitors are unaffected in gfi1b morphants (mean±SE, t test, p<0.05, n=5).

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
la781Tg + MO1-gfi1aa standard conditions 20-25 somites erythroid progenitor cell decreased amount, abnormal
20-25 somites hematopoietic progenitor cell differentiation decreased functionality, abnormal
20-25 somites myeloid lineage restricted progenitor cell increased amount, abnormal
la781Tg + MO1-gfi1b standard conditions 20-25 somites erythroid progenitor cell normal amount, normal
20-25 somites myeloid lineage restricted progenitor cell normal amount, normal
20-25 somites primitive hemopoiesis process quality, normal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 373(2), Cooney, J.D., Hildick-Smith, G.J., Shafizadeh, E., McBride, P.F., Carroll, K.J., Anderson, H., Shaw, G.C., Tamplin, O.J., Branco, D.S., Dalton, A.J., Shah, D.I., Wong, C., Gallagher, P.G., Zon, L.I., North, T.E., and Paw, B.H., Teleost growth factor independence (gfi) genes differentially regulate successive waves of hematopoiesis, 431-441, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.