Gene
gfi1aa
- ID
- ZDB-GENE-050522-534
- Name
- growth factor independent 1A transcription repressor a
- Symbol
- gfi1aa Nomenclature History
- Previous Names
-
- gfi1.1
- gfi1a
- zgc:113277 (1)
- Type
- protein_coding_gene
- Location
- Chr: 2 Mapping Details/Browsers
- Description
- Predicted to enable DNA-binding transcription repressor activity, RNA polymerase II-specific and RNA polymerase II cis-regulatory region sequence-specific DNA binding activity. Acts upstream of or within hemangioblast cell differentiation and primitive erythrocyte differentiation. Predicted to be active in nucleoplasm. Is expressed in several structures, including artery; hematopoietic cell; inner ear; lateral line system; and mesoderm. Used to study myelodysplastic syndrome. Human ortholog(s) of this gene implicated in acute myeloid leukemia and severe congenital neutropenia 2. Orthologous to human GFI1 (growth factor independent 1 transcriptional repressor).
- Genome Resources
- Note
- None
- Comparative Information
-
- All Expression Data
- 25 figures from 12 publications
- Cross-Species Comparison
- High Throughput Data
- Thisse Expression Data
-
- IMAGE:7158523 (10 images)
Wild Type Expression Summary
- All Phenotype Data
- 23 figures from 7 publications
- Cross-Species Comparison
- Alliance
Phenotype Summary
Mutations
| Allele | Type | Localization | Consequence | Mutagen | Supplier |
|---|---|---|---|---|---|
| qmc551Gt | Transgenic insertion | Intron 1 | Unknown | DNA | |
| sa9056 | Allele with one point mutation | Unknown | Premature Stop | ENU | |
| sa11633 | Allele with one point mutation | Exon 4 | Premature Stop | ENU | |
| sa16850 | Allele with one point mutation | Unknown | Premature Stop | ENU | |
| szy5 | Allele with one deletion | Unknown | Unknown | TALEN |
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| Targeting Reagent | Created Alleles | Citations |
|---|---|---|
| CRISPR1-gfi1aa | Zebrafish Nomenclature Committee | |
| CRISPR2-gfi1aa | Tamaoki et al., 2023 | |
| CRISPR3-gfi1aa | Tamaoki et al., 2023 | |
| CRISPR4-gfi1aa | Tamaoki et al., 2023 | |
| CRISPR5-gfi1aa | Tamaoki et al., 2023 | |
| MO1-gfi1aa | N/A | (4) |
| MO2-gfi1aa | N/A | (2) |
| TALEN1-gfi1aa | (5) |
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Human Disease
| Disease Ontology Term | Multi-Species Data | OMIM Term | OMIM Phenotype ID |
|---|---|---|---|
| severe congenital neutropenia 2 | Alliance | Neutropenia, severe congenital 2, autosomal dominant | 613107 |
| ?Neutropenia, nonimmune chronic idiopathic, of adults | 607847 |
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| Human Disease | Fish | Conditions | Citations |
|---|---|---|---|
| myelodysplastic syndrome | gfi1aaszy5/szy5; i114Tg/i114Tg | standard conditions | Wu et al., 2021 |
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Domain, Family, and Site Summary
Domain Details Per Protein
| Protein | Length | Zinc finger C2H2 superfamily | Zinc finger C2H2-type |
|---|---|---|---|
|
UniProtKB:Q501T1
|
385 |
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| Type | Name | Annotation Method | Has Havana Data | Length (nt) | Analysis |
|---|---|---|---|---|---|
| mRNA |
gfi1aa-201
(1)
|
Ensembl | 1,464 nt | ||
| mRNA |
gfi1aa-202
(1)
|
Ensembl | 1,607 nt |
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Interactions and Pathways
No data available
Plasmids
No data available
| Construct | Regulatory Region | Coding Sequence | Species | Tg Lines | Citations |
|---|---|---|---|---|---|
| Tg(gfi1aa:GFP) |
|
| 1 | (7) | |
| Tg(hsp70l:gfi1aa-EGFP) |
|
| 1 | Wu et al., 2022 |
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| Relationship | Marker Type | Marker | Accession Numbers | Citations |
|---|---|---|---|---|
| Contained in | BAC | CH73-243I7 | ZFIN Curated Data | |
| Contained in | BAC | DKEY-63K7 | ZFIN Curated Data | |
| Encodes | EST | IMAGE:7158523 | Thisse et al., 2004 | |
| Encodes | cDNA | MGC:113277 | ZFIN Curated Data | |
| Encodes | cDNA | MGC:192642 | ZFIN Curated Data |
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| Type | Accession # | Sequence | Length (nt/aa) | Analysis |
|---|---|---|---|---|
| RNA | RefSeq:NM_001020776 (1) | 1480 nt | ||
| Genomic | GenBank:CR450845 (1) | 232604 nt | ||
| Polypeptide | UniProtKB:Q501T1 (1) | 385 aa |
- Heng, J., Shi, B., Zhou, J.Y., Zhang, Y., Ma, D., Yang, Y.G., Liu, F. (2023) Cpeb1b-mediated cytoplasmic polyadenylation of shha mRNA modulates zebrafish definitive hematopoiesis. Proceedings of the National Academy of Sciences of the United States of America. 120:e2212212120e2212212120
- Shi, T., Beaulieu, M.O., Saunders, L.M., Fabian, P., Trapnell, C., Segil, N., Crump, J.G., Raible, D.W. (2023) Single-cell transcriptomic profiling of the zebrafish inner ear reveals molecularly distinct hair cell and supporting cell subtypes. eLIFE. 12:
- Tamaoki, J., Maeda, H., Kobayashi, I., Takeuchi, M., Ohashi, K., Gore, A., Bonkhofer, F., Patient, R., Weinstein, B.M., Kobayashi, M. (2023) LSD1 promotes the egress of hematopoietic stem and progenitor cells into the bloodstream during the endothelial-to-hematopoietic transition. Developmental Biology. 501:92-103
- Casey, M.J., Call, A.M., Thorpe, A.V., Jette, C.A., Engel, M.E., Stewart, R.A. (2022) The scaffolding function of LSD1/KDM1A reinforces a negative feedback loop to repress stem cell gene expression during primitive hematopoiesis. iScience. 26:105737105737
- Reuter, A.S., Stern, D., Bernard, A., Goossens, C., Lavergne, A., Flasse, L., Von Berg, V., Manfroid, I., Peers, B., Voz, M.L. (2022) Identification of an evolutionarily conserved domain in Neurod1 favouring enteroendocrine versus goblet cell fate. PLoS Genetics. 18:e1010109
- Song, Y., Chen, W., Zhu, B., Ge, W. (2022) Disruption of Epidermal Growth Factor Receptor but Not EGF Blocks Follicle Activation in Zebrafish Ovary. Frontiers in cell and developmental biology. 9:750888
- Wu, M., Chen, Q., Li, J., Xu, Y., Lian, J., Liu, Y., Meng, P., Zhang, Y. (2022) Gfi1aa/Lsd1 Facilitates Hemangioblast Differentiation Into Primitive Erythrocytes by Targeting etv2 and sox7 in Zebrafish. Frontiers in cell and developmental biology. 9:786426
- Gao, S., Wang, Z., Wang, L., Wang, H., Yuan, H., Liu, X., Chen, S., Chen, Z., de Thé, H., Zhang, W., Zhang, Y., Zhu, J., Zhou, J. (2021) Irf2bp2a regulates terminal granulopoiesis through proteasomal degradation of Gfi1aa in zebrafish. PLoS Genetics. 17:e1009693
- Wu, M., Xu, Y., Li, J., Lian, J., Chen, Q., Meng, P., Lu, T., Xie, H., Zhang, W., Xu, J., Zhang, Y. (2021) Genetic and epigenetic orchestration of Gfi1aa-Lsd1-cebpα in zebrafish neutrophil development. Development (Cambridge, England). 148(17)
- Heng, J., Lv, P., Zhang, Y., Cheng, X., Wang, L., Ma, D., Liu, F. (2020) Rab5c-mediated endocytic trafficking regulates hematopoietic stem and progenitor cell development via Notch and AKT signaling. PLoS Biology. 18:e3000696
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