ZFIN ID: ZDB-FIG-130128-3
Torregroza et al., 2012 - Regulation of a Vascular Plexus by gata4 Is Mediated in Zebrafish through the Chemokine sdf1a. PLoS One   7(10):e46844 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Prim-15
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Prim-15

Fig. 3 The gata4 morphant CHT fails to develop fenestrated vascular structures.

Shown are higher resolution images of control (A) and two independent examples of morphant embryos (B,C) imaged at 32 hpf by confocal microscopy, as the caudal plexus is forming. While vasculogenesis initiates normally, and intersegmental vessels sprout equivalently, angiogenesis in the CHT of control embryos generates a vascular plexus containing open fenestrated structures, indicated by the asterisks in A, that are missing in gata4 morphants.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP sd2Tg; y1Tg control Prim-15 caudal vein plexus IFL
Prim-15 intersegmental vessel IFL
Prim-15 vascular endothelium IFL
sd2Tg; y1Tg + MO1-gata4 standard conditions Prim-15 caudal vein plexus IFL
Prim-15 intersegmental vessel IFL
Prim-15 vascular endothelium IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
sd2Tg; y1Tg + MO1-gata4 standard conditions Prim-15 caudal vein plexus malformed, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One