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Fig. 3 The gata4 morphant CHT fails to develop fenestrated vascular structures.
Shown are higher resolution images of control (A) and two independent examples of morphant embryos (B,C) imaged at 32 hpf by confocal microscopy, as the caudal plexus is forming. While vasculogenesis initiates normally, and intersegmental vessels sprout equivalently, angiogenesis in the CHT of control embryos generates a vascular plexus containing open fenestrated structures, indicated by the asterisks in A, that are missing in gata4 morphants.
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