ZFIN ID: ZDB-FIG-130128-7
Torregroza et al., 2012 - Regulation of a Vascular Plexus by gata4 Is Mediated in Zebrafish through the Chemokine sdf1a. PLoS One   7(10):e46844 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Protruding-mouth

Fig. 7 Depletion of sdf1a rescues the neuromast and circulation defects in the gata4 morphant.

A: Shown are representative embryos at 72 hpf (n >50, multiple independent experiments) following incubation with 4-Di-2-Asp to detect neuromasts. For comparison with control embryos (left panels) embryos were injected with the gata4 morpholino and half the clutch was left alone (middle panels), while the other half was subsequently injected with the sdf1a morpholino (right panels). The lower panels are the fluorescence images of the corresponding brightfield panels. The examples shown are representative of the rescue for the lateral line in 34% of co-injected embryos (using 0.25 ng of the sdf1a morpholino). B: Shown are representative embryos at 72 hpf (n >50, multiple independent experiments) derived from the tg(gata1:dsRed) reporter line. For comparison with control embryos (left panels) embryos were injected with the gata4 morpholino and half the clutch was left alone (middle panels), while the other half was subsequently injected with the sdf1a morpholino (right panels). The lower panels are the fluorescence images of the corresponding brightfield panels. The rescue of circulation into the CHT plexus in the tail was observed in 82% of co-injected embryos (using 2 ng of the sdf1a morpholino). White arrowheads indicate the caudal vein in the CHT. Views are lateral, anterior to the left.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
DsRed sd2Tg control Protruding-mouth blood cell IFL
sd2Tg + MO1-gata4 standard conditions Protruding-mouth blood cell IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-gata4 standard conditions Protruding-mouth neuromast decreased amount, abnormal
Protruding-mouth posterior lateral line lacks parts or has fewer parts of type neuromast, abnormal
sd2Tg + MO1-gata4 standard conditions Protruding-mouth caudal fin blood circulation decreased process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One