ZFIN ID: ZDB-FIG-130128-2
Torregroza et al., 2012 - Regulation of a Vascular Plexus by gata4 Is Mediated in Zebrafish through the Chemokine sdf1a. PLoS One   7(10):e46844 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Prim-5 to Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Long-pec to Protruding-mouth

Fig. 2 The vascular morphology of the CHT is disrupted in the gata4 morphant embryo.

Shown are representative embryos (n >50) derived from tg(gata1:dsred; fli1:egfp) double transgenic reporter fish. The green signal shows the vascular endothelium, while the red signal shows the circulation of blood cells, which are moving so quickly that individual cells are not seen. At 28 hpf (A,B) the blood circulates to the caudal end of the developing CHT (the caudal end is indicated by white arrows) in both control uninjected (A) and gata4 morphant (B) embryos. However, by 72 hpf (C,D), the vascular plexus is much more developed in the control (C) compared to the morphant (D). In addition, the blood circulation is restricted in the morphant, failing to move into the more caudal regions, as indicated in panels C and D by the white brackets that mark the distance from the caudal end of the CHT to the most caudal position of circulating cells (indicated by the white arrows). The block to plexus formation can be seen clearly at 48 hpf (E,F), when in the control embryos (E) the dorsal aorta and caudal vein are well separated to create a fenestrated space (this space is indicated by double-headed arrows), which is not seen in the morphant (F). MO = morphant. Views are lateral, anterior to the left.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
DsRed sd2Tg; y1Tg control Prim-5 blood cell IFL
Long-pec blood cell IFL
Protruding-mouth blood cell IFL
sd2Tg; y1Tg + MO1-gata4 standard conditions Prim-5 blood cell IFL
Long-pec blood cell IFL
Protruding-mouth blood cell IFL
EGFP sd2Tg; y1Tg control Prim-5 intersegmental vessel IFL
Prim-5 vascular endothelium IFL
Long-pec caudal vein plexus IFL
Long-pec dorsal aorta IFL
Long-pec intersegmental vessel IFL
Long-pec vascular endothelium IFL
Protruding-mouth caudal vein plexus IFL
Protruding-mouth intersegmental vessel IFL
Protruding-mouth vascular endothelium IFL
sd2Tg; y1Tg + MO1-gata4 standard conditions Prim-5 intersegmental vessel IFL
Prim-5 vascular endothelium IFL
Long-pec caudal vein plexus IFL
Long-pec dorsal aorta IFL
Long-pec intersegmental vessel IFL
Long-pec vascular endothelium IFL
Protruding-mouth caudal vein plexus IFL
Protruding-mouth intersegmental vessel IFL
Protruding-mouth vascular endothelium IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
sd2Tg; y1Tg + MO1-gata4 standard conditions Long-pec caudal vein plexus malformed, abnormal
Protruding-mouth caudal fin blood circulation decreased process quality, abnormal
Protruding-mouth caudal vein plexus malformed, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One