ZFIN ID: ZDB-FIG-130123-58
Schweitzer et al., 2013 - Sim1a and Arnt2 contribute to hypothalamo-spinal axon guidance by regulating Robo2 activity via a Robo3-dependent mechanism. Development (Cambridge, England)   140(1):93-106 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Observed In:
Stage: Protruding-mouth

Fig. 5 Robo3 is required for lateral positioning of otpb:gfp-positive longitudinal axons. Dorsal views of confocal hindbrain z-projections (72 hpf) are shown. Anterior is towards the left. (A,B) In twt/+ embryos, otpb:gfp longitudinal axons in the midbrain (A, arrowheads) and hindbrain (A, arrows) grow by a defined distance towards the midline. Arrows in B indicate Mauthner neuron somata. (C,D) In twt/twt embryos, otpb:gfp longitudinal axons project more laterally in the midbrain (C, arrowheads) and hindbrain (C, arrows). (E) Quantification of the separation between otpb:gfp longitudinal axons in mid- and hindbrain (double-headed arrows, II and V in B) and between nucMLF somata (I, see asterisks in B), MLF axons (IV) or MA neurons (III) in twt/+ or twt/twt embryos. (F,G) In sim1a MO-injected twt/+ embryos, otpb:gfp longitudinal axons are medially displaced (F, arrows). (H,I) In sim1a MO-injected twt/twt embryos, otpb:gfp longitudinal axons project more laterally (H, arrows). (J) Quantification of the distance between MA neurons (II, marked in G) and vagal axons (IV in H), or of the distance between otpb:gfp longitudinal axons (I in H and III in G) in sim1a MO-injected twt/+ and twt/twt embryos. Arrowheads in B,D,G,I indicate pathfinding of Mauthner neurons in twt/+ and twt/twt embryos. *P<0.001. Scale bar: 50 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP robo3tw204/+; zc49Tg standard conditions Protruding-mouth diencephalic nucleus axon IHC
robo3tw204/+; zc49Tg + MO2-sim1a standard conditions Protruding-mouth diencephalic nucleus axon IHC
robo3tw204/tw204; zc49Tg standard conditions Protruding-mouth diencephalic nucleus axon IHC
robo3tw204/tw204; zc49Tg + MO2-sim1a standard conditions Protruding-mouth diencephalic nucleus axon IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab-3A10 IHC robo3tw204/+; zc49Tg standard conditions Protruding-mouth Mauthner neuron
IHC Protruding-mouth Mauthner neuron axon
IHC robo3tw204/+; zc49Tg + MO2-sim1a standard conditions Protruding-mouth Mauthner neuron
IHC Protruding-mouth Mauthner neuron axon
IHC robo3tw204/tw204; zc49Tg standard conditions Protruding-mouth Mauthner neuron
IHC Protruding-mouth Mauthner neuron axon
IHC robo3tw204/tw204; zc49Tg + MO2-sim1a standard conditions Protruding-mouth Mauthner neuron
IHC Protruding-mouth Mauthner neuron axon
Phenotype Details
Fish Conditions Stage Phenotype
robo3tw204/tw204; zc49Tg standard conditions Protruding-mouth diencephalic nucleus axon displaced to hindbrain lateral region, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development