ZFIN ID: ZDB-FIG-130123-60
Schweitzer et al., 2013 - Sim1a and Arnt2 contribute to hypothalamo-spinal axon guidance by regulating Robo2 activity via a Robo3-dependent mechanism. Development (Cambridge, England)   140(1):93-106 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Long-pec
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Long-pec

Fig. 7 Dcc-mediated attraction causes medial displacement of otpb:gfp longitudinal axons after sim1a knockdown. Dorsal views of hindbrain confocal z-projections of 48 hpf otpb:gfp embryos. Anterior is towards the left. (A-D) GFP-positive longitudinal axons are medially displaced towards the midline (A, arrows) after co-injection of sim1a MO and control MO in otpb:gfp embryos. Medial displacement of longitudinal otpb:gfp axons (C, arrows) is reduced after combined injection of sim1a MO and dcc MO. (E) Quantification of the distance between otpb:gfp-positive longitudinal axons (I) or of the distance between Mauthner neurons (II) of indicated treatments. Arrowheads in B,D indicate normal midline crossing of MA neurons. *P<0.001; n.s., not significant. Scale bar: 50 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP zc49Tg + MO2-dcc + MO2-sim1a standard conditions Long-pec diencephalic nucleus axon IHC
zc49Tg + MO2-sim1a standard conditions Long-pec diencephalic nucleus axon IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab-3A10 IHC zc49Tg + MO2-dcc + MO2-sim1a standard conditions Long-pec Mauthner neuron
IHC Long-pec Mauthner neuron axon
IHC zc49Tg + MO2-sim1a standard conditions Long-pec Mauthner neuron
IHC Long-pec Mauthner neuron axon
Phenotype Details
Fish Conditions Stage Phenotype
zc49Tg + MO2-sim1a standard conditions Long-pec diencephalic nucleus axon displaced to hindbrain medial region, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development