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ZIRC
ZFIN ID: ZDB-FIG-130123-56
Schweitzer et al., 2013 - Sim1a and Arnt2 contribute to hypothalamo-spinal axon guidance by regulating Robo2 activity via a Robo3-dependent mechanism. Development (Cambridge, England)   140(1):93-106 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Observed In:
Stage: Protruding-mouth

Fig. 3 Lateral positioning of longitudinal axons derived from otpb- and hypocretin-positive neurons is altered in ast homozygous mutants. Dorsal views of hindbrain confocal z-projections of otpb:gfp;hcrt:tdtomato double transgenic 72 hpf embryos. (A-F) In ast/ast embryos longitudinal projections of otpb:gfp (D,F; arrows in D) and hcrt:tdtomato (E,F; arrowheads in E) axons are shifted towards the midline compared with ast/+ siblings. (G) Quantification of the distance between otpb:gfp and hcrt:tdtomato HTS axons at the anterior-posterior level of Mauthner neurons, and of the distance between Mauthner neurons. ***P<0.0001; n.s., not significant. Scale bar in A for A-F: 50 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP robo2ti272z/+; m1163Tg; zc49Tg standard conditions Protruding-mouth diencephalic nucleus axon IHC
robo2ti272z/ti272z; m1163Tg; zc49Tg standard conditions Protruding-mouth diencephalic nucleus axon IHC
Tomato robo2ti272z/+; m1163Tg; zc49Tg standard conditions Protruding-mouth hypocretin-secreting neuron axon IHC
robo2ti272z/ti272z; m1163Tg; zc49Tg standard conditions Protruding-mouth hypocretin-secreting neuron axon IHC
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
robo2ti272z/ti272z; m1163Tg; zc49Tg standard conditions Protruding-mouth diencephalic nucleus axon displaced to hindbrain medial region, abnormal
Protruding-mouth hypocretin-secreting neuron axon displaced to hindbrain medial region, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development