header logo image header logo text
Downloads Login
Research
General Information
ZIRC
ZFIN ID: ZDB-FIG-130123-61
Schweitzer et al., 2013 - Sim1a and Arnt2 contribute to hypothalamo-spinal axon guidance by regulating Robo2 activity via a Robo3-dependent mechanism. Development (Cambridge, England)   140(1):93-106 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Protruding-mouth

Fig. 8 Medial displacement phenotype of otpb:gfp longitudinal HTS axons is not affected by depletion of sim1a in ast/ast; twt/+ and ast/ast; twt/twt embryos. Dorsal views of hindbrain confocal z-projections of 72 hpf otpb:gfp embryos. Anterior is towards the left. (A,B) otpb:gfp HTS axons (arrows) in a wild-type sibling. (C-H) Phenotypic strength of medial displacement of otpb:gfp-positive longitudinal axons is similar in ast/ast; twt/twt embryos injected with control MO (C, arrows), and in ast/ast; twt/+ (E, arrows) or ast/ast; twt/twt (G, arrows) sim1a morphant embryos. Arrowheads in B,D,F,H indicate pathfinding of Mauthner neurons. (I) Quantification of the distance between of otpb:gfp longitudinal axons and vagal axons, and of the distance between Mauthner neurons in ast/ast; twt/twt embryos injected with control MO, and in ast/ast; twt/+ or ast/ast; twt/twt embryos injected with sim1a MO. n.s., not significant. Scale bar: 50 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP robo2ti272z/ti272z; robo3tw204/+; zc49Tg + MO2-sim1a standard conditions Protruding-mouth diencephalic nucleus axon IHC
robo2ti272z/ti272z; robo3tw204/tw204; zc49Tg control Protruding-mouth diencephalic nucleus axon IHC
robo2ti272z/ti272z; robo3tw204/tw204; zc49Tg + MO2-sim1a standard conditions Protruding-mouth diencephalic nucleus axon IHC
zc49Tg standard conditions Protruding-mouth diencephalic nucleus axon IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab-3A10 IHC robo2ti272z/ti272z; robo3tw204/+; zc49Tg + MO2-sim1a standard conditions Protruding-mouth Mauthner neuron
IHC Protruding-mouth Mauthner neuron axon
IHC robo2ti272z/ti272z; robo3tw204/tw204; zc49Tg control Protruding-mouth Mauthner neuron
IHC Protruding-mouth Mauthner neuron axon
IHC robo2ti272z/ti272z; robo3tw204/tw204; zc49Tg + MO2-sim1a standard conditions Protruding-mouth Mauthner neuron
IHC Protruding-mouth Mauthner neuron axon
IHC zc49Tg + MO3-arnt2 standard conditions Protruding-mouth Mauthner neuron
IHC Protruding-mouth Mauthner neuron axon
Phenotype Details
Fish Conditions Stage Phenotype
robo2ti272z/ti272z; robo3tw204/+; zc49Tg + MO2-sim1a standard conditions Protruding-mouth diencephalic nucleus axon displaced to hindbrain medial region, abnormal
robo2ti272z/ti272z; robo3tw204/tw204; zc49Tg control Protruding-mouth diencephalic nucleus axon displaced to hindbrain medial region, abnormal
robo2ti272z/ti272z; robo3tw204/tw204; zc49Tg + MO2-sim1a standard conditions Protruding-mouth diencephalic nucleus axon displaced to hindbrain medial region, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development