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ZFIN ID: ZDB-FIG-130123-59
Schweitzer et al., 2013 - Sim1a and Arnt2 contribute to hypothalamo-spinal axon guidance by regulating Robo2 activity via a Robo3-dependent mechanism. Development (Cambridge, England)   140(1):93-106 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Condition:
Anatomical Terms:
Stage Range: Long-pec to Protruding-mouth
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage Range: Long-pec to Protruding-mouth

Fig. 6 Ectopic expression of robo3a.1 is sufficient to induce medial displacement of HTS axons. (A-F′′,H-M′′) Dorsal views of confocal z-projections of brain regions of 48 hpf (A-F′′) and 72 hpf (H-M′′) embryos. Anterior is towards the to left. (A-B′′,H-I′′) Heat-shock treatment does not affect pathfinding of otpb:gfp or hcrt:tdtomato longitudinal axon in control embryos. (C-D′′,J-K′′) Longitudinal otpb:gfp or hcrt:tdtomato axons are medially displaced after heat shock-induced overexpression of robo3a.1. (E-F′′,L-M′′) Pathfinding of otpb:gfp or hcrt:tdtomato longitudinal axons is normal after overexpression of robo3b.1. (G,N) Quantification of the number of otpb:gfp or hcrt:tdtomato longitudinal axons in defined outer or inner sections. Brackets in B,B′,D,F, I,I′,K,M, indicate outer and inner sections as defined by Mauthner neuron somata (outer section) and the area in between (inner section) used for quantification. Asterisks and plus signs indicate longitudinal HTS axons in outer and inner sections, respectively. Scale bars: in A, 50 μm for A,C,E,H,J,L; in B, 25 μm for B-B′′,D-D′′,F-F′′,I-I′′,K-K′′,M-M′′.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP m1217Tg; zc49Tg heat shock Long-pec diencephalic nucleus axon IHC
m1218Tg; zc49Tg heat shock Long-pec diencephalic nucleus axon IHC
zc49Tg heat shock Long-pec diencephalic nucleus axon IHC
Tomato m1163Tg heat shock Protruding-mouth hypocretin-secreting neuron axon IHC
m1163Tg; m1217Tg heat shock Protruding-mouth hypocretin-secreting neuron axon IHC
m1163Tg; m1218Tg heat shock Protruding-mouth hypocretin-secreting neuron axon IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab-3A10 IHC m1163Tg heat shock Protruding-mouth Mauthner neuron
IHC Protruding-mouth Mauthner neuron axon
IHC m1163Tg; m1217Tg heat shock Protruding-mouth Mauthner neuron
IHC Protruding-mouth Mauthner neuron axon
IHC m1163Tg; m1218Tg heat shock Protruding-mouth Mauthner neuron
IHC Protruding-mouth Mauthner neuron axon
IHC m1217Tg; zc49Tg heat shock Long-pec Mauthner neuron
IHC Long-pec Mauthner neuron axon
IHC m1218Tg; zc49Tg heat shock Long-pec Mauthner neuron
IHC Long-pec Mauthner neuron axon
IHC zc49Tg heat shock Long-pec Mauthner neuron
IHC Long-pec Mauthner neuron axon
Phenotype Details
Fish Conditions Stage Phenotype
m1163Tg; m1218Tg heat shock Protruding-mouth hypocretin-secreting neuron axon displaced to hindbrain medial region, abnormal
m1218Tg; zc49Tg heat shock Long-pec diencephalic nucleus axon displaced to hindbrain medial region, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development