ZFIN ID: ZDB-FIG-130123-54
Schweitzer et al., 2013 - Sim1a and Arnt2 contribute to hypothalamo-spinal axon guidance by regulating Robo2 activity via a Robo3-dependent mechanism. Development (Cambridge, England)   140(1):93-106 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Protruding-mouth

Fig. 1 Sim1a and Arnt2 act synergistically during longitudinal HTS axon guidance. Dorsal views of hindbrain confocal z-projections of otpb:gfp transgenic embryos at 72 hpf. Anterior is towards the left. (A-H) Mediolateral positioning of otpb:gfp axons (arrows) after indicated treatments. otpb:gfp hindbrain neurons (asterisks in A,C,E,G) do not contribute to longitudinal projections. (I) Quantification of the distance between otpb:gfp axons (I), of the distance between MA neurons (II) or between otpb:gfp hindbrain neurons (III). *P<0.0001 for sim1a MO 1.0 ng, arnt2 MO 4.5 ng, arnt2/sim1a MO 1.0/4.5 ng versus control MO 4.5 ng. Scale bar: 50 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP zc49Tg control Protruding-mouth diencephalic nucleus axon IHC
Protruding-mouth hindbrain neuron IHC
Protruding-mouth hindbrain nucleus axon IHC
zc49Tg + MO2-sim1a standard conditions Protruding-mouth diencephalic nucleus axon IHC
Protruding-mouth hindbrain neuron IHC
Protruding-mouth hindbrain nucleus axon IHC
zc49Tg + MO2-sim1a + MO3-arnt2 standard conditions Protruding-mouth diencephalic nucleus axon IHC
Protruding-mouth hindbrain neuron IHC
Protruding-mouth hindbrain nucleus axon IHC
zc49Tg + MO3-arnt2 standard conditions Protruding-mouth diencephalic nucleus axon IHC
Protruding-mouth hindbrain neuron IHC
Protruding-mouth hindbrain nucleus axon IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab-3A10 IHC zc49Tg control Protruding-mouth Mauthner neuron
IHC Protruding-mouth Mauthner neuron axon
IHC zc49Tg + MO2-sim1a standard conditions Protruding-mouth Mauthner neuron
IHC Protruding-mouth Mauthner neuron axon
IHC zc49Tg + MO2-sim1a + MO3-arnt2 standard conditions Protruding-mouth Mauthner neuron
IHC Protruding-mouth Mauthner neuron axon
IHC zc49Tg + MO3-arnt2 standard conditions Protruding-mouth Mauthner neuron
IHC Protruding-mouth Mauthner neuron axon
Phenotype Details
Fish Conditions Stage Phenotype
zc49Tg + MO2-sim1a standard conditions Protruding-mouth diencephalic nucleus axon displaced to hindbrain medial region, abnormal
zc49Tg + MO2-sim1a + MO3-arnt2 standard conditions Protruding-mouth diencephalic nucleus axon displaced to hindbrain medial region, abnormal
zc49Tg + MO3-arnt2 standard conditions Protruding-mouth diencephalic nucleus axon displaced to hindbrain medial region, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development