FIGURE

Fig. 8

ID

ZDB-FIG-121004-23

Publication

An et al., 2012 - The zebrafish sf3b1(b460) mutant reveals differential requirements for the sf3b1 pre-mRNA processing gene during neural crest development

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Fig. 8

Early development of ectodermal and mesodermal derivatives in sf3b1^b460 mutants. (A,B) Whole-mount in situ hybridization with islet-1 antisense RNA probes shows that the number of Rohon-Beard precursor cells (arrows) is decreased in sf3b1^b460mutants (dorsal view, anterior to top). (C-F) Whole-mount in situ hybridization with islet-2 antisense RNA probe shows deficiency in the development of Rohon-Beard sensory neurons (arrows), whereas the development of primary motor neurons is comparatively less affected in mutant embryos (asterisks). (G,H) Dorsal view of spinal cords labeled with anti-acetylated tubulin (Anti Ac-tub), anterior to left, showing that the Rohon-Beard sensory neuron phenotype persists at later stages in sf3b1^b460 mutants. K-N: Induction of the neural plate border occurs normally in sf3b1^b460 mutants. Whole-mount in situ hybridization with msxB (K,L) and dlx3 (M,N). (I,J;O,P) The derivatives from paraxial mesoderm and chordamesoderm are not affected in sf3b1^b460mutants. (I,J) Lateral view of whole-mount in situ with ntl, anterior to left. (O,P) Dorsal view of double in situ with crestin (red) and myoD (blue), anterior to top.

Expression Data

Genes:	crestin dlx3b isl1a isl2a msx1b tbxta
Fish:	AB sf3b1^b460/b460
Anatomical Terms:	neural crest cell neural plate notochord primary motor neuron Rohon-Beard neuron
Stage Range:	1-4 somites to 26+ somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	sf3b1^b460/b460
Observed In:	neural plate morphogenesis notochord primary motor neuron Rohon-Beard neuron somite
Stage Range:	1-4 somites to 26+ somites

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Int. J. Dev. Biol.