FIGURE

Fig. 6

ID

ZDB-FIG-121004-21

Publication

An et al., 2012 - The zebrafish sf3b1(b460) mutant reveals differential requirements for the sf3b1 pre-mRNA processing gene during neural crest development

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Fig. 6

Whole-mount in situ hybridization with sf3b1 antisense probe and sense probes in wildtype embryos. (A,C) sf3b1 is ubiquitously expressed at 0.5 hpf and 14 hpf. (E,G) The expression of sf3b1 appears elevated in the brain, neural tube and ventral trunk region at 19 hpf and 24 hpf (lateral view, anterior to left). (B,D,F) sf3b1 sense controls. (H) RT-PCR products using total RNA from 0.5hpf (lane1) and 24hpf (lane2) wildtype embryos as templates and Sf3b1aa(46)F and Sf3b1aa(579)R as primers. (I) Western blot analysis shows Sf3b1 protein present maternally. Lane1: protein from 24 hpf wildtype embryos as control. Lane2: protein from 1 hpf wildtype embryos.

Expression Data

Gene:	sf3b1
Fish:	AB
Anatomical Terms:	brain neural tube dorsal region trunk ventral region whole organism
Stage Range:	2-cell to Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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