FIGURE

Fig. S9

ID
ZDB-FIG-120803-24
Publication
McCarroll et al., 2012 - Graded levels of Pax2a and Pax8 regulate cell differentiation during sensory placode formation
Other Figures
All Figure Page
Back to All Figure Page
Fig. S9

Overactivation of Wnt signaling severely reduces or blocks EB ganglia formation. Confocal projection images of the otic vesicle and EB ganglia at 50 hpf. Tg(phox2b:EGFP)w37 embryos were treated with 2.5 and 5.0 μM BIO beginning 11 hpf, then washed out at 24 hpf. Embryos were allowed to develop until 50 hpf, at which time they were assessed for EB ganglion formation by EGFP expression and for otic vesicle formation by DAPI labeling. (A-C) Whereas DMSO-treated control embryos had normal development of the otic vesicle and EB ganglia, approximately half of 2.5 μM BIO-treated embryos had severely reduced EB ganglia and the remaining half had no EBs. (D) BIO (5.0 μM)-treated embryos resulted in a complete loss of EBs. Scale bar: 25 μm.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development