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Fig. S6 Increased RARE:YFP expression in response to exogenous RA after crabp2a and crabp2b knockdown. Live Tg(RARE-gata2:NTD-eYFP)Id1 transgenics, dorsal views of the hindbrain, anterior to the left. (A,B) Expression extends just anterior to the level of somite 1 (s1) in DMSO-treated controls (A) and extends further anteriorly <100 μm after treatment during gastrulation with 1 nM exogenous RA (B). (C,D) crabp2a/crabp2b knockdown alone slightly reduces Tg(RARE-gata2:NTD-eYFP)Id1 expression (C), but when combined with 1 nm RA expression expands throughout the CNS (D). ′o′ indicates approximate location of the otic vesicle.