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ZFIN ID: ZDB-FIG-120216-31
Balczerski et al., 2012 - Analysis of Sphingosine-1-phosphate signaling mutants reveals endodermal requirements for the growth but not dorsoventral patterning of jaw skeletal precursors. Developmental Biology   361(2):230-241 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Term:
Stage: Prim-25
PHENOTYPE:
Fish:
Observed In:
Stage: Prim-25

Fig. 5

DV gene expression in endoderm mutants. (A–P) Double fluorescent in situ hybridizations show the expression of dlx2a (red) with hand2, dlx3b, msxe, or jag1b (green) in the pharyngeal arches (numbered) at 36 hpf. In wild types, hand2 (A) was restricted to the ventral-most domain, dlx3b (E) and msxe (I) to a DV-intermediate domain, and jag1b (M) to the dorsal arches. hand2 expression remained restricted to ventral arch CNCCs in sox32 embryos, although ectopic hand2 expression was seen in ectoderm overlying the second pouch (arrow) (B, n = 12/12). The hand2 expression domain remained ventrally restricted but was expanded in size in s1pr2 (D, n = 10/10), and spns2b1110 (C, n = 10/10) embryos. The DV-intermediate expression of dlx3b was normal in sox32 (F, n = 4/4), s1pr2 (G, n = 10/10), and spns2b1110 (H, n = 5/5) embryos, as was expression of msxe in sox32 (J, n = 7/7), s1pr2 (L, n = 11/11), and spns2b1110 (K, n = 5/5) embryos. jag1b expression was reduced but remained dorsal-restricted in sox32 embryos (N, n = 5/5) and was unaffected in s1pr2 (O, n = 5/5) and spns2b1110 (P, n = 6/6) embryos. (Q–T) Tracings of the dlx2a-expressing arches (solid lines) and the DV expression borders (dashed lines) of hand2 (Q), dlx3b (R), msxe (S), or jag1b (T) show slight expansion of hand2 expression but no change in dlx3b, msxe, or jag1b expression in two classes of s1pr2 and spns2b1110 mutants. For wild types, dlx2a expression is light gray, with DV gene expression domains in darker gray. The mandibular arch was expanded (green lines) in some embryos (C, G, K, O; 47% of s1pr2 and 53% of spns2b1110) and was abnormally shaped and/or anteriorly truncated (red lines) in other embryos (D, H, L, P; 47% of s1pr2 and 16% of spns2b1110). Scale bar = 50 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
dlx2a WT standard conditions Prim-25 pharyngeal arch ISH
s1pr2m93/m93 standard conditions Prim-25 pharyngeal arch ISH
sox32ta56/ta56 standard conditions Prim-25 pharyngeal arch ISH
spns2b1110/b1110 standard conditions Prim-25 pharyngeal arch ISH
dlx3b WT standard conditions Prim-25 pharyngeal arch ISH
s1pr2m93/m93 standard conditions Prim-25 pharyngeal arch ISH
sox32ta56/ta56 standard conditions Prim-25 pharyngeal arch ISH
spns2b1110/b1110 standard conditions Prim-25 pharyngeal arch ISH
hand2 WT standard conditions Prim-25 pharyngeal arch ISH
s1pr2m93/m93 standard conditions Prim-25 pharyngeal arch ISH
sox32ta56/ta56 standard conditions Prim-25 pharyngeal arch ISH
spns2b1110/b1110 standard conditions Prim-25 pharyngeal arch ISH
jag1b WT standard conditions Prim-25 pharyngeal arch ISH
s1pr2m93/m93 standard conditions Prim-25 pharyngeal arch ISH
sox32ta56/ta56 standard conditions Prim-25 pharyngeal arch ISH
spns2b1110/b1110 standard conditions Prim-25 pharyngeal arch ISH
msx1a WT standard conditions Prim-25 pharyngeal arch ISH
s1pr2m93/m93 standard conditions Prim-25 pharyngeal arch ISH
sox32ta56/ta56 standard conditions Prim-25 pharyngeal arch ISH
spns2b1110/b1110 standard conditions Prim-25 pharyngeal arch ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
s1pr2m93/m93 standard conditions Prim-25 dorsal/ventral pattern formation process quality, normal
Prim-25 mandibular arch skeleton shape, abnormal
Prim-25 mandibular arch skeleton size, abnormal
sox32ta56/ta56 standard conditions Prim-25 dorsal/ventral pattern formation process quality, normal
Prim-25 mandibular arch skeleton shape, abnormal
Prim-25 mandibular arch skeleton size, abnormal
spns2b1110/b1110 standard conditions Prim-25 dorsal/ventral pattern formation process quality, normal
Prim-25 mandibular arch skeleton shape, abnormal
Prim-25 mandibular arch skeleton size, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 361(2), Balczerski, B., Matsutani, M., Castillo, P., Osborne, N., Stainier, D.Y., and Crump, J.G., Analysis of Sphingosine-1-phosphate signaling mutants reveals endodermal requirements for the growth but not dorsoventral patterning of jaw skeletal precursors, 230-241, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.