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ZFIN ID: ZDB-FIG-120216-29
Balczerski et al., 2012 - Analysis of Sphingosine-1-phosphate signaling mutants reveals endodermal requirements for the growth but not dorsoventral patterning of jaw skeletal precursors. Developmental Biology   361(2):230-241 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Prim-25
PHENOTYPE:
Fish:
Observed In:
Stage: Prim-25

Fig. 3

Facial ectoderm gene expression in endoderm mutants. (A–T) In situ hybridizations at 36 hpf. In wild types, pitx2ca (A), shha (E), and fgf8a (I) are expressed in oral ectoderm and edn1 (M) and bmp4 (Q) in aboral ectoderm. pitx2ca expression in the oral ectoderm (arrowheads) is present but shifted anteriorly in sox32 (B, n = 6/6), s1pr2 (C, n = 11/11), and spns2b1110 (D, n = 4/4) embryos. The expression of shha in the oral ectoderm is lost in all mutants, whereas endoderm expression (arrows) is absent in sox32 mutants (F, n = 7/7), variably posteriorly displaced (G, n = 10/15) or disorganized (see 4, n = 3/15) in s1pr2 mutants, and variably posteriorly displaced (H, n = 6/7) or disorganized (not shown, n = 1/7) in spns2b1110 mutants. fgf8a expression is reduced in sox32 mutants (J, n = 6/6), reduced (K, n = 4/10) or disorganized (not shown, n = 4/10) in s1pr2 mutants, and reduced (not shown, n = 13/27) or disorganized (L, n = 14/27) in spns2b1110 mutants. edn1 expression in the first two arches (white lines) is present but disorganized in sox32 (N, n = 8/8), s1pr2 (O, n = 6/6), and spns2b1110 (P, n = 5/5) mutants. bmp4 expression is also disorganized in sox32 (R, n = 6/6), s1pr2 (S, n = 7/7), and spns2b1110 (T, n = 5/5) mutants. Outlines of the oral ectoderm and first two pouches are shown for wild types. Scale bar = 50 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
bmp4 WT standard conditions Prim-25 ectoderm ISH
s1pr2m93/m93 standard conditions Prim-25 ectoderm ISH
sox32ta56/ta56 standard conditions Prim-25 ectoderm ISH
spns2b1110/b1110 standard conditions Prim-25 ectoderm ISH
edn1 WT standard conditions Prim-25 ectoderm ISH
s1pr2m93/m93 standard conditions Prim-25 ectoderm ISH
sox32ta56/ta56 standard conditions Prim-25 ectoderm ISH
spns2b1110/b1110 standard conditions Prim-25 ectoderm ISH
fgf8a WT standard conditions Prim-25 oral region ectoderm ISH
s1pr2m93/m93 standard conditions Prim-25 Not Detected oral region ectoderm ISH
sox32ta56/ta56 standard conditions Prim-25 oral region ectoderm ISH
spns2b1110/b1110 standard conditions Prim-25 oral region ectoderm ISH
pitx2 WT standard conditions Prim-25 oral region ectoderm ISH
s1pr2m93/m93 standard conditions Prim-25 oral region ectoderm ISH
sox32ta56/ta56 standard conditions Prim-25 oral region ectoderm ISH
spns2b1110/b1110 standard conditions Prim-25 oral region ectoderm ISH
shha WT standard conditions Prim-25 endoderm ISH
Prim-25 oral region ectoderm ISH
s1pr2m93/m93 standard conditions Prim-25 ectoderm ISH
Prim-25 endoderm ISH
Prim-25 Not Detected oral region ectoderm ISH
sox32ta56/ta56 standard conditions Prim-25 Not Detected ectoderm ISH
Prim-25 Not Detected endoderm ISH
Prim-25 Not Detected oral region ectoderm ISH
spns2b1110/b1110 standard conditions Prim-25 ectoderm ISH
Prim-25 endoderm ISH
Prim-25 Not Detected oral region ectoderm ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
s1pr2m93/m93 standard conditions Prim-25 oral ectoderm disorganized, abnormal
Prim-25 oral ectoderm hypoplastic, abnormal
Prim-25 oral ectoderm mislocalised anteriorly, abnormal
Prim-25 pharyngeal ectoderm disorganized, abnormal
Prim-25 pharyngeal ectoderm increased area, abnormal
Prim-25 pharyngeal endoderm disorganized, abnormal
Prim-25 pharyngeal endoderm mislocalised posteriorly, abnormal
sox32ta56/ta56 standard conditions Prim-25 oral ectoderm disorganized, abnormal
Prim-25 oral ectoderm hypoplastic, abnormal
Prim-25 oral ectoderm mislocalised anteriorly, abnormal
Prim-25 pharyngeal ectoderm disorganized, abnormal
Prim-25 pharyngeal ectoderm increased area, abnormal
Prim-25 pharyngeal endoderm aplastic, abnormal
spns2b1110/b1110 standard conditions Prim-25 oral ectoderm disorganized, abnormal
Prim-25 oral ectoderm hypoplastic, abnormal
Prim-25 oral ectoderm mislocalised anteriorly, abnormal
Prim-25 pharyngeal ectoderm disorganized, abnormal
Prim-25 pharyngeal ectoderm increased area, abnormal
Prim-25 pharyngeal endoderm disorganized, abnormal
Prim-25 pharyngeal endoderm mislocalised posteriorly, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 361(2), Balczerski, B., Matsutani, M., Castillo, P., Osborne, N., Stainier, D.Y., and Crump, J.G., Analysis of Sphingosine-1-phosphate signaling mutants reveals endodermal requirements for the growth but not dorsoventral patterning of jaw skeletal precursors, 230-241, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.