ZFIN ID: ZDB-FIG-120209-19
Zhang et al., 2012 - Zebrafish Models for Dyskeratosis Congenita Reveal Critical Roles of p53 Activation Contributing to Hematopoietic Defects through RNA Processing. PLoS One   7(1):e30188 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: Prim-15 to Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Prim-15 to Day 4

Fig. 3

Activation of p53 pathway and rescue of hematopoietic defects in zebrafish dkc1 and nola1 deficiency.

(A) and (C) Expression of genes of p53 pathway was up-regulated in both dkc1 morphant (A) and nola1 mutant (C). Expression level of wild type embryos of related genes was normalized as 1.0. (B) Knockdown of p53 by injection of p53 MO rescued the reduction of HSC in dkc1 morphants (compare c with e, d with f). C-myb expression at 3 dpf (a, c and e); runx1 expression at 30 hpf (b, d and f). (D) Red blood cells were restored in nola1 and p53 double mutatant embryos (red arrow in ad). 25 embryos at 4 dpf were examined for each group. And the results shown here were representative for three independent experiments.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
baxa WT + MO1-dkc1 standard conditions Protruding-mouth whole organism RTPCR
gar1zf303Tg/zf303Tg standard conditions Protruding-mouth whole organism RTPCR
ccng1 WT + MO1-dkc1 standard conditions Protruding-mouth whole organism RTPCR
gar1zf303Tg/zf303Tg standard conditions Protruding-mouth whole organism RTPCR
myb WT standard conditions Protruding-mouth hematopoietic stem cell ISH
WT + MO1-dkc1 standard conditions Protruding-mouth hematopoietic stem cell ISH
WT + MO1-dkc1 + MO4-tp53 standard conditions Protruding-mouth hematopoietic stem cell ISH
runx1 WT standard conditions Prim-15 hematopoietic stem cell ISH
WT + MO1-dkc1 standard conditions Prim-15 hematopoietic stem cell ISH
WT + MO1-dkc1 + MO4-tp53 standard conditions Prim-15 hematopoietic stem cell ISH
tp53 WT + MO1-dkc1 standard conditions Protruding-mouth whole organism RTPCR
gar1zf303Tg/zf303Tg standard conditions Protruding-mouth whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-dkc1 standard conditions Prim-15 hematopoietic stem cell decreased amount, abnormal
Protruding-mouth signal transduction by p53 class mediator increased process quality, abnormal
WT + MO1-dkc1 + MO4-tp53 standard conditions Prim-15 hematopoietic stem cell normal amount, normal
Protruding-mouth hematopoietic stem cell normal amount, normal
gar1zf303Tg/zf303Tg standard conditions Protruding-mouth signal transduction by p53 class mediator increased process quality, abnormal
Day 4 nucleate erythrocyte decreased amount, abnormal
tp53zdf1/zdf1 standard conditions Day 4 nucleate erythrocyte normal amount, normal
tp53zdf1/zdf1; gar1zf303Tg/zf303Tg standard conditions Day 4 nucleate erythrocyte normal amount, normal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One