Fig. 1

Molecular and phenotype analysis of dkc1 morphant and nola1 retroviral insertion mutatant of zebrafish.

(A) Splicing MO targeting dkc1 sequence caused the inclusion of intron 4 into mRNA (a). Semi-quantitive PCR data showed an increase of 1.5 kb in the PCR product in dkc1 morphants (b). According to Real Time PCR result, expression of dkc1 was diminished by more than 90% when high dose (15 ng) of MO was injected (c). Pictures of embryos at 3 dpf (d and e) and 5 dpf (f and g) showed smaller eyes and smaller head (red arrows in d, e, f and g) in dkc1 morphants. Compared to wild type embryos, dkc1 morphants developed edema, and had fewer red blood cells (red arrowhead in d, e, f and g). d and f: wild type controls; e and g: dkc1 morphants. (B) A retroviral insertion in exon1 of the nola1 gene led to the phenotype of smaller eyes, smaller head, and cardiac edema in nola1 homozygous mutant. Red arrow indicated the smaller eyes and smaller head, and red arrowhead showed edema in nola1 homozygous mutant at 3 dpf (a and b) and 5 dpf (c and d). Microinjection of nola1 mRNA (f), but not eGFP mRNA (e) can rescue the mutant phenotype of nola1 homozygous mutants at 5 dpf (red arrow and arrowhead in e and f). a and c: wild type siblings; b, d, e and f: nola1 homozygous mutants. Expression of nola1 was reduced more than 90% in nola1 homozygous mutants (g). All the pictures of embryos are lateral view with anterior to the left.