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ZFIN ID: ZDB-FIG-120131-34
Wang et al., 2011 - Tfap2a and Foxd3 regulate early steps in the development of the neural crest progenitor population. Developmental Biology   360(1):173-85 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Anatomical Terms:
Stage: 75%-epiboly

Fig. 4 Bmp signaling is expanded in mob;mos embryos. (A–H) bmp2b (A–D) and bmp4 (E–H) expression domains (blue) are expanded in mob and mos embryos and further enlarged in mob;mos embryos at 8 hpf. Embryos are oriented according to the position of the prechordal plate marker hgg1 (red). (I–P) The expression domain of chordin is reduced in mob and mos embryos and further diminished in mob;mos embryos. Lateral views I–L; Vegetal views M–P. Arrowheads point to the limits of the signal-positive chordin domain in vegetal view, dorsal side to the right (M–P). (Q–X) The phospho-Smad1/5/8-positive (P-Smad) domain representing active Bmp signaling is increased in mob and mos embryos and further expanded in mob;mos embryos. Embryos in Q–T are shown in lateral view dorsal side to the right, and in U–X in vegetal view, dorsal side to the right. Arrowheads (U–X) point to the limits of the P-Smad domain.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
bmp2b WT control 75%-epiboly non neural ectoderm ISH
foxd3m188/m188 control 75%-epiboly non neural ectoderm ISH
tfap2am610/m610 control 75%-epiboly non neural ectoderm ISH
tfap2am610/m610; foxd3m188/m188 control 75%-epiboly non neural ectoderm ISH
bmp4 WT control 75%-epiboly non neural ectoderm ISH
foxd3m188/m188 control 75%-epiboly non neural ectoderm ISH
tfap2am610/m610 control 75%-epiboly non neural ectoderm ISH
tfap2am610/m610; foxd3m188/m188 control 75%-epiboly non neural ectoderm ISH
chrd WT control 75%-epiboly margin dorsal region ISH
foxd3m188/m188 control 75%-epiboly margin dorsal region ISH
tfap2am610/m610 control 75%-epiboly margin dorsal region ISH
tfap2am610/m610; foxd3m188/m188 control 75%-epiboly margin dorsal region ISH
ctslb WT control 75%-epiboly prechordal plate ISH
foxd3m188/m188 control 75%-epiboly prechordal plate ISH
tfap2am610/m610 control 75%-epiboly prechordal plate ISH
tfap2am610/m610; foxd3m188/m188 control 75%-epiboly prechordal plate ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-smad IHC WT control 75%-epiboly unspecified
IHC foxd3m188/m188 control 75%-epiboly unspecified
IHC tfap2am610/m610 control 75%-epiboly unspecified
IHC tfap2am610/m610; foxd3m188/m188 control 75%-epiboly unspecified
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 360(1), Wang, W.D., Melville, D.B., Montero-Balaguer, M., Hatzopoulos, A.K., and Knapik, E.W., Tfap2a and Foxd3 regulate early steps in the development of the neural crest progenitor population, 173-85, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.