ZFIN ID: ZDB-FIG-120131-32
Wang et al., 2011 - Tfap2a and Foxd3 regulate early steps in the development of the neural crest progenitor population. Developmental Biology   360(1):173-85 Full text @ Dev. Biol.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Bud to Day 5

Fig. 2 Apoptosis is not the primary cause of the mob;mos double mutant phenotype. (A, B) Lateral view of acridine orange (AO) staining of wild-type (WT) (A) and mob;mos (B) embryos at 10 hpf. (C, D) Dorsal view of TUNEL assay in WT (C) and mob;mos (D) embryos revealing more apoptotic cells in the neural plate region of mob;mos embryos at 10 hpf. (E) Quantification of TUNEL positive cells in the neural plate region of WT, mob, foxd3-MO-injected, and foxd3-knockdown mob embryos. Each spot represents one embryo. n signifies the number of embryos counted. Error bars represent standard deviation. Data analyzed by Student′s t-test, *P < 0.05, **P < 0.01, and ***P < 0.001. (F–J) Suppression of apoptosis by knock-down of p53 does not rescue the phenotype in mob;mos embryos. Lateral view of anti-Hu immunostaining at 3 dpf in WT (F) and mob;mos (G) embryos injected with p53 MO. Ventral view of Alcian blue staining of the head at 5 dpf of WT (H) and mob;mos (I) embryos injected with p53 MO. The proportion of embryos in a double-heterozygote mob;mos cross showing absence of pharyngeal arch skeleton does not change upon injection of p53 MO (J). n signifies the number of embryos counted.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
tfap2am610/m610; foxd3m188/m188 standard conditions Bud apoptotic process increased occurrence, abnormal
tfap2am610/m610; foxd3m188/m188 + MO4-tp53 standard conditions Protruding-mouth whole organism lacks all parts of type dorsal root ganglion, abnormal
Protruding-mouth whole organism lacks all parts of type enteric nervous system neuron, abnormal
Day 5 whole organism lacks all parts of type pharyngeal arch cartilage, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 360(1), Wang, W.D., Melville, D.B., Montero-Balaguer, M., Hatzopoulos, A.K., and Knapik, E.W., Tfap2a and Foxd3 regulate early steps in the development of the neural crest progenitor population, 173-85, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.