ZFIN ID: ZDB-FIG-120131-35
Wang et al., 2011 - Tfap2a and Foxd3 regulate early steps in the development of the neural crest progenitor population. Developmental Biology   360(1):173-85 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Term:
Stage: 75%-epiboly
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Day 5

Fig. 5 Expression of dkk1, a Wnt signaling inhibitor, is increased in mob;mos embryos, whereas expression of canonical Wnt targets is suppressed. (A–D) Dorsal view of embryos stained with dkk1 riboprobes in wild-type (WT) (A), mob (B), mos (C), and mob;mos (D) at 75% epiboly (8 hpf). dkk1-positive cells are slightly more in mob or mos, but are highly increased in mob;mos embryos. Insets in A and D show transverse sections through the dkk1-expressing region in the mesendoderm. (E) Quantitative PCR analysis of canonical Wnt signaling targets axin2 and sp5l mRNA levels in wild-type embryos and tfap2a-and foxd3-MO knockdown morphants at 8 hpf. Error bars represent standard deviation. Data analyzed by Student′s t-test, **, P < 0.01 and ***, P < 0.001. (F–I) Morpholino-mediated knockdown of dkk1 partially restores pharyngeal arch formation in mob;mos embryos. Ventral view of Alcian blue-stained heads at 5 dpf showing the craniofacial skeleton of wild-type (F), dkk1 morphants (G), mob;mos (H), and dkk1-MO knockdown mob;mos (I) embryos. Head cartilage in mob;mos embryos injected with dkk1-MO is partially restored (red arrows). (J) Percentage of mob;mos embryos with no pharyngeal skeleton and rescued pharyngeal arches with or without (Control) dkk1-MO injection. n indicates the number of embryos examined, N the number of experiments performed. Data analyzed by Chi-squared test; ***, p < 0.001.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
dkk1b WT control 75%-epiboly axial hypoblast ISH
foxd3m188/m188 control 75%-epiboly axial hypoblast ISH
tfap2am610/m610 control 75%-epiboly axial hypoblast ISH
tfap2am610/m610; foxd3m188/m188 control 75%-epiboly axial hypoblast ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO2-dkk1b standard conditions Day 5 pharyngeal arch morphology, normal
Day 5 pharyngeal arch present, normal
tfap2am610/m610; foxd3m188/m188 control Day 5 whole organism lacks all parts of type pharyngeal arch cartilage, abnormal
tfap2am610/m610; foxd3m188/m188 + MO2-dkk1b standard conditions Day 5 pharyngeal arch decreased size, abnormal
Day 5 pharyngeal arch malformed, abnormal
Day 5 pharyngeal arch morphology, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 360(1), Wang, W.D., Melville, D.B., Montero-Balaguer, M., Hatzopoulos, A.K., and Knapik, E.W., Tfap2a and Foxd3 regulate early steps in the development of the neural crest progenitor population, 173-85, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.