ZFIN ID: ZDB-FIG-120131-31
Wang et al., 2011 - Tfap2a and Foxd3 regulate early steps in the development of the neural crest progenitor population. Developmental Biology   360(1):173-85 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 5-9 somites to Prim-5
PHENOTYPE:
Fish:
Observed In:
Stage Range: 5-9 somites to Day 5

Fig. 1 mob;mos double mutant embryos exhibit complete absence of neural crest and its derivatives. (A–D) Lateral view of wild-type (WT) (A), mob (B), mos (C), and mob;mos (D) live embryos at 5 dpf. In mob and mos embryos the Meckel′s cartilage (m) is pointing ventrally (arrows in B,C). In mob;mos embryos, the ventral part of the head (arrow in D) is ventrally concave and skin pigmentation is absent. (E–H) Ventral view of Alcian blue-stained heads showing the craniofacial skeleton of WT (E), mob (F), mos (G), and mob;mos (H) embryos at 5 dpf. Pharyngeal cartilage elements corresponding to the second and posterior arches are reduced in mob and mos embryos; in mob;mos embryos, all pharyngeal cartilage is absent, and the neurocranium is deformed. bh, basihyal; cb 1–5, ceratobranchials 1–5; ch, ceratohyal; ep, ethmoid plate; hs, hyosymplectic; m, Meckel′s cartilage; pch, parachordal; pq, palatoquadrate; tr, trabecula. (I–L) Lateral view of trunk regions of WT (I), mob (J), mos (K), and mob;mos (L) embryos stained with anti-Hu antibody to label dorsal root ganglia (DRG) and enteric neurons (en) at 3 dpf. DRG and enteric neurons are reduced and scattered in mob and mos embryos (arrows in J,K) and absent in mob;mos embryos. In contrast, Rohon–Beard neurons are present in double mutants (arrow in L). (M–R) Migratory neural crest cells are absent in mob;mos embryos. In situ hybridization analysis for genes expressed in migratory neural crest cells in WT (M, O, Q) and mob;mos (N, P, R) embryos at 24 hpf. Embryos in (O, P) are flattened and shown in dorsal view, the rest in lateral views. crestin (M, N) expression in the migratory neural crest is completely absent in mob;mos embryos. dlx2a (O, P) is expressed in the telencephalon (tel), diencephalon, and migratory neural crest streams in the head of wild-type embryos, but absent in mob;mos embryos. sox10 (Q, R) is expressed in non-ectomesenchymal neural crest cells and otic vesicles (ov) of wild-type embryos, while in mob;mos embryos, sox10 is only expressed in otic vesicles. (S–V) Dorsal view of WT (S), mob (T), mos (U) and mob;mos (V) embryos showing expression of snail1b at 12 hpf in premigratory neural crest, which is completely absent in mob;mos embryos.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
crestin WT control Prim-5 neural crest cell ISH
tfap2am610/m610; foxd3m188/m188 control Prim-5 Not Detected neural crest cell ISH
dlx2a WT control Prim-5 diencephalon ISH
Prim-5 neural crest cell ISH
Prim-5 telencephalon ISH
tfap2am610/m610; foxd3m188/m188 control Prim-5 diencephalon ISH
Prim-5 Not Detected neural crest cell ISH
Prim-5 telencephalon ISH
snai1b WT control 5-9 somites neural crest ISH
foxd3m188/m188 control 5-9 somites neural crest ISH
tfap2am610/m610 control 5-9 somites neural crest ISH
tfap2am610/m610; foxd3m188/m188 control 5-9 somites Not Detected neural crest ISH
sox10 WT control Prim-5 neural crest ISH
Prim-5 otic vesicle ISH
tfap2am610/m610; foxd3m188/m188 control Prim-5 Not Detected neural crest ISH
Prim-5 otic vesicle ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
foxd3m188/m188 control Protruding-mouth dorsal root ganglion neuron decreased amount, abnormal
Protruding-mouth enteric nervous system neuron decreased amount, abnormal
Protruding-mouth peripheral nervous system neuron distributed, abnormal
Day 5 pharyngeal arch 1 structure, normal
Day 5 pharyngeal arch 2 skeleton decreased size, abnormal
Day 5 pharyngeal arch 2 skeleton morphology, abnormal
Day 5 pharyngeal arch 3-7 skeleton decreased size, abnormal
Day 5 pharyngeal arch 3-7 skeleton morphology, abnormal
tfap2am610/m610 control Protruding-mouth dorsal root ganglion neuron decreased amount, abnormal
Protruding-mouth enteric nervous system neuron decreased amount, abnormal
Protruding-mouth peripheral nervous system neuron distributed, abnormal
Day 5 pharyngeal arch 1 structure, normal
Day 5 pharyngeal arch 2 skeleton decreased size, abnormal
Day 5 pharyngeal arch 2 skeleton morphology, abnormal
Day 5 pharyngeal arch 3-7 skeleton decreased size, abnormal
Day 5 pharyngeal arch 3-7 skeleton morphology, abnormal
tfap2am610/m610; foxd3m188/m188 control 5-9 somites neural crest cell development disrupted, abnormal
5-9 somites whole organism lacks all parts of type neural crest, abnormal
Prim-5 neural crest cell development disrupted, abnormal
Prim-5 whole organism lacks all parts of type neural crest cell, abnormal
Protruding-mouth whole organism lacks all parts of type dorsal root ganglion, abnormal
Protruding-mouth whole organism lacks all parts of type enteric nervous system neuron, abnormal
Day 5 head concave, abnormal
Day 5 neurocranium deformed, abnormal
Day 5 neurocranium lacks all parts of type ethmoid cartilage, abnormal
Day 5 neurocranium lacks all parts of type trabecula cranii, abnormal
Day 5 pigmentation arrested, abnormal
Day 5 whole organism lacks all parts of type pharyngeal arch cartilage, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 360(1), Wang, W.D., Melville, D.B., Montero-Balaguer, M., Hatzopoulos, A.K., and Knapik, E.W., Tfap2a and Foxd3 regulate early steps in the development of the neural crest progenitor population, 173-85, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.