ZFIN ID: ZDB-FIG-111129-28
Goudevenou et al., 2011 - Def6 Is Required for Convergent Extension Movements during Zebrafish Gastrulation Downstream of Wnt5b Signaling. PLoS One   6(10):e26548 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Bud to Prim-5
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Bud to Prim-5

Fig. 5

The def6 MO-mediated knockdown phenotype induces CE movement defects.

Uninjected (wt) or embryos injected with def6 MO were fixed at tail-bud stage and in situ hybridisations were carried out with probes to hgg1 and dlx3 (A,B). ImageJ software was utilised to analyse the staining patterns, measuring the posterior shift of the hgg1 staining (red double-headed arrow) in relation to the arc formed by dlx3 expression (yellow dotted arc) (C), and measuring the width of the dlx3 staining (blue double-headed arrow) at a constant distance (1/4 of the embryo width) from the dlx3 arc when the embryo was positioned dorsally (D). (E and F) The measured distances were plotted as the average posterior shift (E) or width (F) as a percentage of the total width of the embryo. Two-tailed Student′s t-tests were carried out between groups indicated, and were of statistical significance (p<0.001; three asterisks). This experiment has been repeated at least three times; a representative experiment is depicted here. Zebrafish embryos uninjected (wt) or injected with def6 MO (2.5 ng) were also stained for hgg1/ntl (G, H; statistical analysis shown in Panel I), ntl (J, K) and myoD (L-O-) expression. Images G, H, L, M are embryos at tail-bud stage. Images J. K show embryos at 24 hpf. Images N, O show embryos at the 10-somite stage. G, H, L-O are dorsal views with anterior to the top; J, K are lateral views with anterior to the left.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
ctslb WT standard conditions Bud polster ISH
WT + MO2-def6a standard conditions Bud polster ISH
dlx3b WT standard conditions Bud anterior neural plate ISH
WT + MO2-def6a standard conditions Bud anterior neural plate ISH
myod1 WT standard conditions Bud adaxial cell ISH
Bud paraxial mesoderm ISH
10-13 somites adaxial cell ISH
10-13 somites somite ISH
WT + MO2-def6a standard conditions Bud adaxial cell ISH
Bud paraxial mesoderm ISH
10-13 somites adaxial cell ISH
10-13 somites somite ISH
tbxta WT standard conditions Bud notochord ISH
Prim-5 notochord ISH
WT + MO2-def6a standard conditions Bud notochord ISH
Prim-5 notochord ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO2-def6a standard conditions Bud convergent extension involved in neural plate elongation disrupted, abnormal
Bud neural plate increased width, abnormal
Bud notochord decreased length, abnormal
Bud notochord increased width, abnormal
Bud paraxial mesoderm adaxial cell increased distance paraxial mesoderm adaxial cell, abnormal
Bud polster mislocalised posteriorly, abnormal
Bud segmental plate increased width, abnormal
10-13 somites convergent extension involved in gastrulation disrupted, abnormal
10-13 somites paraxial mesoderm formation disrupted, abnormal
10-13 somites segmental plate curved, abnormal
10-13 somites somite morphology, abnormal
Prim-5 convergent extension involved in axis elongation disrupted, abnormal
Prim-5 notochord decreased length, abnormal
Prim-5 notochord undulate, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One