Fig. S2

Injection of def6 ATG MO or splice MO result in embryos with a similar phenotype. (A) Injections with 25 ng def6 ATG MO leads to embryos with a reduced body axis (black arrowheads) when compared to wild-type controls. Two-tailed Student t-tests indicate a significant (p<0.001; three asterisks) increase in the angle between the most anterior and posterior embryonic structures of def6morphants in comparison to WT embryos. (B) Morphological analysis showing the similarities between ATG MO- (ii, v, vii) and splice MO- (iii, vi, viii) injected embryos compared to WT siblings (i, iv). Both MOs result in embryos with a reduced body axis at tail-bud stage (black arrowheads). At 24 hpf, both MOs result in morphants with head (red arrows), somite (yellow arrows) and tail (green arrows) defects as well as heart oedema (purple arrowheads) and an undulated notochord (black arrows). Images v and vi show moderately affected embryos (v; 39/71 ATG MO and vi: 32/83 splice MO injected embryos). Images vii and viii show severely affected embryos (vii; 32/71 def6 ATG MO- and viii; 51/83 def6 splice MO-injected embryos) at 24hpf.