Fig. 9

Synergy between def6 and wnt11 MO-mediated knockdown results in severe phenotype.

Zebrafish embryos were injected with wnt11 MO (1.5 ng) and def6 MO (1.5 ng) separately and together. Development was assessed at different stages. (A–C) Tail-bud stage (10 hpf), the anterior-most structure is indicated with an arrowhead. (D–F) 28 hpf, co-injection with def6 and wnt11 MOs results in no forebrain structures anterior to the eyes (D′–F′) the same embryos are shown from the front; note complete fusion of the eyes in the double-knockdown embryos. (G–I) 3 dpf, double knockdown embryos have their eyes completely fused in comparison to def6 or wnt11 MO-injected embryos. (J) The phenotypes of 28 hpf embryos were scored morphologically into 4 categories. Representative embryos of normal (blue box), mild (purple box), moderate (green box) and severely affected (red box) morphants are shown. (K) The phenotypes of the embryos from three independent experiments were scored and the percentages of normal (blue bar), mild (purple bar), moderate (green bar) and severe (red bar) are indicated.